caa a22 4500 463236521 CHVBK 20180405153259.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11240-007-9220-9 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9220-9 Glutathione metabolism and antioxidant responses during Eleutherococcus senticosus somatic embryo development in a bioreactor [Elektronische Daten] [A. Shohael, M. Ali, E. Hahn, K. Paek] Compared to non-embryogenic callus, proembryonic mass, globular, and heart-shaped embryos of Eleutherococcus senticosus had higher levels of endogenous reduced glutathione (GSH). GSH content declined during the course of the embryo development (torpedo and cotyledon). Similarly, glutathione reductase that is involved in the recycling of GSH providing a constant intracellular level of GSH was also higher in globular and heart-shaped embryos. The transient increase in GSH contents also correlated with the changes in measured γ-glutamylcysteine synthetase activity over the same period. The endogenous levels of oxidized glutathione showed similar trend during development of the somatic embryos, whereas it declined in maturing somatic embryos. A pronounced increase in glutathione-S-transferase, glutathione peroxidase, catalase, and guaiacol peroxidase activity was observed during somatic embryo maturation. Ascorbate-glutathione cycle enzymes (ascorbate peroxidase; dehydroascorbate reductase and monodehydroascorbate reductase) activities also induced indicated that antioxidant enzymes played an important role during embryo development. These results suggested that the coordinated up-regulations of the antioxidant enzymes and glutathione redox system provide protection during somatic embryo development in E. senticosus. Antioxidant responses through alterations of the glutathione redox systems, have been described in the present studies have a significant role in somatic embryo development. Springer Science+Business Media B.V., 2007 Bioreactor nationallicence Eleutherococcus senticosus nationallicence Enzymes nationallicence γ-glutamylcysteine synthetase nationallicence Glutathione nationallicence Somatic embryogenesis nationallicence APX : Ascorbate peroxidase (EC 1.11.1.11) nationallicence CAT : Catalase (EC 1.11.1.6) nationallicence 2, 4 D : 2, 4-dichlorophenoxyaceticacid nationallicence DHAR : Dehydroascorbate reductase (EC 1.8.5.1) nationallicence DHA : Dehydroascorbate nationallicence γ-GCS : Glutamylcysteine synthetase nationallicence GST : Glutathione-S-transferase (EC 2.5.1.18) nationallicence GPx : Glutathione peroxidase (EC 1.11.1.12) nationallicence GR : Glutathione reductase (EC 1.6.4.2) nationallicence G-POD : Guaiacol peroxidase (EC 1.11.1.7) nationallicence MDHAR : Monodehydroascorbate reductase (EC 1.6.5.4) nationallicence GSH : Reduced glutathione nationallicence GSSG : Oxidized glutathione nationallicence Shohael A. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, Korea aut Ali M. Metabolic Regulation Laboratory, Food Biotechnology Division, National Food Research Institute, 305-8642, Tsukuba, Ibaraki, Kannondai, Japan aut Hahn E. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, Korea aut Paek K. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, Korea aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 121-129 0167-6857 89:2-3<121 2007 89 11240 https://doi.org/10.1007/s11240-007-9220-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9220-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shohael A. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, Korea aut NATIONALLICENCE 700 1- Ali M. Metabolic Regulation Laboratory, Food Biotechnology Division, National Food Research Institute, 305-8642, Tsukuba, Ibaraki, Kannondai, Japan aut NATIONALLICENCE 700 1- Hahn E. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, Korea aut NATIONALLICENCE 700 1- Paek K. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, Korea aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 121-129 0167-6857 89:2-3<121 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer