caa a22 4500 463236548 CHVBK 20180405153259.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s11240-007-9276-6 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9276-6 Improved genetic transformation protocol for cork oak ( Quercus suber L.) [Elektronische Daten] [Rubén Álvarez, Ricardo Ordás] An optimized protocol for Agrobacterium tumefaciens-mediated transformation of mature Quercus suberL. embryogenic masses is reported. In this work several variables were tested. Plant genotype, explant type and time elapsed between the last subculture and inoculation, i.e. the explant preculture period, were found to be very important. Interaction between inoculum density and cocultivation period influenced the transformation efficiency as well. A transformation efficiency (i.e. percentage of the inoculated explants that yielded independent transgenic embryogenic lines) of up to 43% was obtained, greatly improving the previously described method for plant transformation of adult-selected cork oak. It was also shown that this protocol could be applied to various genotypes. Springer Science+Business Media B.V., 2007 Agrobacterium nationallicence Fagaceae nationallicence Somatic embryogenesis nationallicence Tree genetic transformation nationallicence GE : number of GUS spots per inoculated explant three weeks after the inoculation nationallicence GUS : β-glucuronidase enzyme nationallicence GUS+ : percentage of Kan+ lines positive for β-glucuronidase nationallicence IG : percentage of inoculated explants showing GUS spots three weeks after the inoculation nationallicence Kan+ : percentage of inoculated explants producing kanamycin-resistant embryos after 4months of culture on selective medium nationallicence MSSH : expression-proliferation medium nationallicence Npt II : neomycin phosphotransferase gene II nationallicence OD600nm : optical density of the bacterial cultures at 600nm nationallicence Gus A : β-glucuronidase gene nationallicence Álvarez Rubén Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, C/. Catedrático Rodrigo Uría s/n, 33011, Oviedo, Spain aut Ordás Ricardo Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, C/. Catedrático Rodrigo Uría s/n, 33011, Oviedo, Spain aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/1(2007-10-01), 45-52 0167-6857 91:1<45 2007 91 11240 https://doi.org/10.1007/s11240-007-9276-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9276-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Álvarez Rubén Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, C/. Catedrático Rodrigo Uría s/n, 33011, Oviedo, Spain aut NATIONALLICENCE 700 1- Ordás Ricardo Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, C/. Catedrático Rodrigo Uría s/n, 33011, Oviedo, Spain aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/1(2007-10-01), 45-52 0167-6857 91:1<45 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer