caa a22 4500 463236572 CHVBK 20180405153259.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s11240-007-9274-8 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9274-8 High-efficiency phosphinothricin-based selection for alfalfa transformation [Elektronische Daten] [Andrew Montague, Asma Ziauddin, Raymond Lee, W. Ainley, Judith Strommer] Despite the significant advantages of using herbicide resistance for selection of genetically engineered plants, alfalfa transformation has relied primarily on selection for antibiotic resistance. In the few studies reporting the use of resistance to the herbicide phosphinothricin (PPT), transformation efficiencies were low. The present investigation describes a PPT-based selection system for alfalfa transformation that uses the phosphinothricin acetyl-transferase (pat) gene as a selectable marker and 5.0mgl−1 of bialaphos as the selective agent. The method achieves transformation efficiencies, measured as the percentage of explants giving rise to one or more transformed plantlets, greater than 50%. These plantlets accumulated detectable amounts of PAT at levels varying from 2 to 1367pgμg−1 total protein. Transformed plants transferred to soil in the greenhouse were phenotypically normal and exhibited resistance to bialaphos leaf painting at 5gl−1 and applications of PPT equivalent to field-level use (0.5kgha−1). Springer Science+Business Media B.V., 2007 Alfalfa nationallicence Bialaphos nationallicence Genetic transformation nationallicence Glufosinate nationallicence Medicago nationallicence pat nationallicence Phosphinothricin nationallicence PPT nationallicence CsVMV : Cassava vein mosaic virus nationallicence GA : Ammonium glufosinate nationallicence GS : Glutamine synthetase nationallicence PAT : Phosphinothricin acetyl-transferase nationallicence PPT : Phosphinothricin nationallicence SAS : SAS/STAT™ statistical software nationallicence Montague Andrew Department of Plant Agriculture, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Ziauddin Asma Department of Plant Agriculture, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Lee Raymond Department of Plant Agriculture, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Ainley W. Dow AgroSciences, 9330 Zionsville Road, 46268, Indianapolis, IN, USA aut Strommer Judith Department of Plant Agriculture, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/1(2007-10-01), 29-36 0167-6857 91:1<29 2007 91 11240 https://doi.org/10.1007/s11240-007-9274-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9274-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Montague Andrew Department of Plant Agriculture, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Ziauddin Asma Department of Plant Agriculture, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Lee Raymond Department of Plant Agriculture, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Ainley W. Dow AgroSciences, 9330 Zionsville Road, 46268, Indianapolis, IN, USA aut NATIONALLICENCE 700 1- Strommer Judith Department of Plant Agriculture, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/1(2007-10-01), 29-36 0167-6857 91:1<29 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer