caa a22 4500 463236580 CHVBK 20180405153259.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s11240-007-9273-9 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9273-9 Assessment of somaclonal variation in Dieffenbachia plants regenerated through indirect shoot organogenesis [Elektronische Daten] [Xiuli Shen, Jianjun Chen, Michael Kane, Richard Henny] The occurrence of somaclonal variation among regenerants derived through indirect shoot organogenesis from leaf explants of three Dieffenbachia cultivars Camouflage, Camille and Star Bright was evaluated. Three types of somaclonal variants (SV1, SV2, and SV3) were identified from regenerated plants of cv. Camouflage, one type from cv. Camille, but none from cv. Star Bright. The three variants had novel and distinct foliar variegation patterns compared to cv. Camouflage parental plants. Additionally, SV1 was taller with a larger canopy and longer leaves than parental plants and SV2. SV2 and SV3 did not produce basal shoots (single stem) but basal shoot numbers between SV1 and parental plants were similar ranging from three to four. The variant type identified from regenerated cv. Camille had lanceolate leaves compared to the oblong leaves of the parent. This variant type also grew taller and had a larger canopy than parental plants. The rates of somaclonal variation were up to 40.4% among regenerated cv. Camouflage plants and 2.6% for regenerated cv. Camille. The duration of callus culture had no effect on somaclonal variation rates of cv. Camouflage as the rates between plants regenerated from 8months to 16months of callus culture were similar. The phenotypes of the identified variants were stable as verified by their progenies after cutting propagation. This study demonstrated the potential for new cultivar development by selecting callus-derived somaclonal variants of Dieffenbachia. Springer Science+Business Media B.V., 2007 Dieffenbachia nationallicence Organogenesis nationallicence Somaclonal variation nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence 2iP : N6-(Δ2-isopentenyl) adenine nationallicence IAA : Indole-3-acetic acid nationallicence TDZ : Thidiazuron nationallicence Shen Xiuli Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-0675, Gainesville, FL, USA aut Chen Jianjun Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-0675, Gainesville, FL, USA aut Kane Michael Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-0675, Gainesville, FL, USA aut Henny Richard Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-0675, Gainesville, FL, USA aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/1(2007-10-01), 21-27 0167-6857 91:1<21 2007 91 11240 https://doi.org/10.1007/s11240-007-9273-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9273-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shen Xiuli Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-0675, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Chen Jianjun Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-0675, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Kane Michael Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-0675, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Henny Richard Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-0675, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/1(2007-10-01), 21-27 0167-6857 91:1<21 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer