caa a22 4500 463236602 CHVBK 20180405153259.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s11240-007-9277-5 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9277-5 Invitro maturation and germination of Orychophragmus violaceus microspores [Elektronische Daten] [Xiaoguang Zhao, Wenguo Wang, Yaodong Li, Jie Xing, Fang Chen, Shenghua Wang] Microspores cultured invitro can be regarded as a system to study gene regulation, cell fate determination and cell differentiation during pollen development as well as an alternative method of genetic transformation in plants. In our study, pollen development and viability in Orychophragmus violaceus invivo were determined and then pollen from the late unicellular stage was cultured invitro. MS liquid medium+White vitamins+2% (V/V) coconut milk+0.5M maltose, pH=7.0 was the most appropriate for invitro culture of Orychophragmus violaceus microspores. With this medium, the rates of in maturation and germination were 19.3% and 4.7%, respectively. Liquid medium with 0.6M maltose+1.6mM boric acid+2.9mM Ca(NO3)2+29.6 μM vitamin B1, pH=7.0 was optimal for germination of pollen matured invivo. The rate of germination was 70.7%. Pollen matured invitro cultured in similar medium exhibited a rate of germination of 62.7%. Hence, the experimental study showed that invitro maturation of microspores is feasible and this experimental system can be applied to further theoretical and practical research. Springer Science+Business Media B.V., 2007 Brassicaceae nationallicence Pollen germination nationallicence Pollen culture nationallicence Viability nationallicence 6-BA : Benzyladenine nationallicence DAPI : 4′,6-diamidino-2-phenylindole nationallicence FDA : Fluorescein diacetate nationallicence Zhao Xiaoguang Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, 610064, Chengdu, Sichuan, P.R. China aut Wang Wenguo Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, 610064, Chengdu, Sichuan, P.R. China aut Li Yaodong Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, 610064, Chengdu, Sichuan, P.R. China aut Xing Jie Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, 610064, Chengdu, Sichuan, P.R. China aut Chen Fang Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, 610064, Chengdu, Sichuan, P.R. China aut Wang Shenghua Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, 610064, Chengdu, Sichuan, P.R. China aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/1(2007-10-01), 53-60 0167-6857 91:1<53 2007 91 11240 https://doi.org/10.1007/s11240-007-9277-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9277-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Xiaoguang Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, 610064, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 700 1- Wang Wenguo Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, 610064, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 700 1- Li Yaodong Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, 610064, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 700 1- Xing Jie Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, 610064, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 700 1- Chen Fang Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, 610064, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 700 1- Wang Shenghua Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, 610064, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/1(2007-10-01), 53-60 0167-6857 91:1<53 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer