caa a22 4500 463236610 CHVBK 20180405153259.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s11240-007-9292-6 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9292-6 A simple method for reducing moisture condensation on Petri dish lids [Elektronische Daten] [Julia Finer, John Finer] Condensation on the lids of Petri dishes, used to culture plant tissues, can often obscure the view of the contents of the dish and interfere with data collection. Under the high humidity conditions that exist in the culture container, a small temperature drop causes water to condense on the inside lid and sides of the container. Mild condensation causes "fogging” while continual or repeated rounds of condensation result in the formation of water droplets. To control condensation in the standard plant tissue culture Petri dish, a simple method was developed whereby the lid of the culture dish was modified, to buffer the lid from temperature fluctuations. Polymer discs, which were the same diameter as the Petri dish lid, were either placed on the top of the lids of existing dishes or surface-sterilized and used in place of the lid. Polymer discs of varying thicknesses and type, and possessing different thermal conductivities, were evaluated for their abilities to reduce the rate of condensation formation. Petri dishes with modified lids were placed under reduced temperature conditions. Condensation, forming on the lids of the dishes was quantified over time using image analysis. Gray value determinations indicated that the thicker polymer discs with the lowest thermal conductivities provided the best protection against condensation. Placement of polymer discs on the top of Petri dishes is a relatively simple method that can be used to buffer the lid from small temperature changes and minimize condensation problems. Springer Science+Business Media B.V., 2007 Condensation problems nationallicence Image analysis nationallicence Insulated lid nationallicence Petri dish design nationallicence Thermal conductivity nationallicence Visibility nationallicence PE : Polyethylene nationallicence PS : Polystyrene nationallicence PC : Polycarbonate nationallicence PG : Plexiglas® nationallicence Finer Julia Department of Horticulture and Crop Science, Plant Molecular Biology and Biotechnology Program, Ohio Agricultural Research and Development Center/The Ohio State University, 1680 Madison Ave., 44691, Wooster, OH, USA aut Finer John Department of Horticulture and Crop Science, Plant Molecular Biology and Biotechnology Program, Ohio Agricultural Research and Development Center/The Ohio State University, 1680 Madison Ave., 44691, Wooster, OH, USA aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 299-304 0167-6857 91:3<299 2007 91 11240 https://doi.org/10.1007/s11240-007-9292-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9292-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Finer Julia Department of Horticulture and Crop Science, Plant Molecular Biology and Biotechnology Program, Ohio Agricultural Research and Development Center/The Ohio State University, 1680 Madison Ave., 44691, Wooster, OH, USA aut NATIONALLICENCE 700 1- Finer John Department of Horticulture and Crop Science, Plant Molecular Biology and Biotechnology Program, Ohio Agricultural Research and Development Center/The Ohio State University, 1680 Madison Ave., 44691, Wooster, OH, USA aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 299-304 0167-6857 91:3<299 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer