caa a22 4500 463236629 CHVBK 20180405153259.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s11240-007-9290-8 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9290-8 TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory ( Cichorium intybus L.) [Elektronische Daten] [Buhara Yucesan, Arzu Turker, Ekrem Gurel] A very efficient and rapid regeneration system via multiple shoot formation was developed for Cichorium intybus L. when leaf explants excised from sterile seedlings were cultured on medium supplemented with different concentrations and combinations of various plant growth regulators. In a comparison of leaf lamina and petiole explants, lamina explants produced over three times more shoots than petiole explants, with a mean of 7.5 shoots compared to 2.4. Of the combinations of KIN/IAA, KIN/NAA, BAP/IAA, or BAP/NAA, 0.5mgl−1 KIN combined with 0.3mgl−1 IAA was the most effective, producing a mean of 19.7 shoots per lamina explant while the control treatment involving no plant growth regulators produced no shoots at all. When either cytokinin was used alone, BAP was found nearly twice more successful than KIN. However, the most effective treatment of all was the combination of 0.01mgl−1 TDZ and 1.0mgl−1 IAA, producing as many as 35.8 shoots per lamina explant. This rate of shoot regeneration is remarkably higher than those previously reported for C. intybus, most likely due to the highly inductive effect of TDZ, which was tested for the first time in this species. Rooting of the shoots was readily achieved on medium containing different concentrations of IAA or IBA. IAA was more effective than IBA and resulted in the highest frequency of shoots that rooted (100%) and mean number of roots per shoot (4.2) when used at 0.5mgl−1. Hardening off process resulted in a production of more than 80% healthy plantlets. Springer Science+Business Media B.V., 2007 Cichorium intybus L nationallicence Lamina explants nationallicence Petiole explants nationallicence Multiple shoot formation nationallicence Invitro regeneration nationallicence TDZ nationallicence Yucesan Buhara Department of Biology, Faculty of Science and Literature, Abant Izzet Baysal University, 14280, Bolu, Turkey aut Turker Arzu Department of Biology, Faculty of Science and Literature, Abant Izzet Baysal University, 14280, Bolu, Turkey aut Gurel Ekrem Department of Biology, Faculty of Science and Literature, Abant Izzet Baysal University, 14280, Bolu, Turkey aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 243-250 0167-6857 91:3<243 2007 91 11240 https://doi.org/10.1007/s11240-007-9290-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9290-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yucesan Buhara Department of Biology, Faculty of Science and Literature, Abant Izzet Baysal University, 14280, Bolu, Turkey aut NATIONALLICENCE 700 1- Turker Arzu Department of Biology, Faculty of Science and Literature, Abant Izzet Baysal University, 14280, Bolu, Turkey aut NATIONALLICENCE 700 1- Gurel Ekrem Department of Biology, Faculty of Science and Literature, Abant Izzet Baysal University, 14280, Bolu, Turkey aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 243-250 0167-6857 91:3<243 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer