caa a22 4500 463236637 CHVBK 20180405153259.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s11240-007-9296-2 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9296-2 Phytodecta fornicata Brüggemann resistance mediated by oryzacystatin II proteinase inhibitor transgene [Elektronische Daten] [Slavica Ninković, Jovanka Miljuš-Đukić, Svetlana Radović, Vesna Maksimović, Jelica Lazarević, Branka Vinterhalter, Mirjana Nešković, Ann Smigocki] Phytodecta fornicata Brüggemann is a serious pest of alfalfa (Medicago sativa L.) that causes significant crop loss in the Balkan peninsula of Europe. We introduced a wound-inducible oryzacystatin II (OCII) gene to alfalfa to evaluate its effect on survival of P.fornicata larvae. Feeding bioassays with second, third and fourth instars were carried out using transgenic plants that were shown to express OCII at 24 and 48h after wounding. Second and third instars were the most sensitive to the ingestion of OCII, whereas no effects were observed with fourth instars. About 80% of the second and third instars died after 2days of feeding on the transgenic plants as compared to 0-40% on the controls. This is the first report that demonstrates significant increase in mortality of P.fornicata on transgenic plants that express a cysteine proteinase inhibitor gene, and this knowledge should lead to the development of effective management strategies for this devastating pest of alfalfa. Springer Science+Business Media B.V., 2007 Alfalfa nationallicence Cystatin nationallicence Leaf beetle nationallicence Second instars nationallicence Transgenic plants nationallicence Ninković Slavica Institute for Biological Research "S. Stanković”, University of Belgrade, Despota Stefana 142, 11060, Belgrade, Serbia aut Miljuš-Đukić Jovanka Institute for Genetics and Genetic Engineering, Bulevar Vojvode Stepe 444a, 11060, Belgrade, Serbia aut Radović Svetlana Institute for Genetics and Genetic Engineering, Bulevar Vojvode Stepe 444a, 11060, Belgrade, Serbia aut Maksimović Vesna Institute for Genetics and Genetic Engineering, Bulevar Vojvode Stepe 444a, 11060, Belgrade, Serbia aut Lazarević Jelica Institute for Biological Research "S. Stanković”, University of Belgrade, Despota Stefana 142, 11060, Belgrade, Serbia aut Vinterhalter Branka Institute for Biological Research "S. Stanković”, University of Belgrade, Despota Stefana 142, 11060, Belgrade, Serbia aut Nešković Mirjana Institute for Biological Research "S. Stanković”, University of Belgrade, Despota Stefana 142, 11060, Belgrade, Serbia aut Smigocki Ann Molecular Plant Pathology Laboratory, USDA-ARS, 20705, Beltsville, MD, USA aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 289-294 0167-6857 91:3<289 2007 91 11240 https://doi.org/10.1007/s11240-007-9296-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9296-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ninković Slavica Institute for Biological Research "S. Stanković”, University of Belgrade, Despota Stefana 142, 11060, Belgrade, Serbia aut NATIONALLICENCE 700 1- Miljuš-Đukić Jovanka Institute for Genetics and Genetic Engineering, Bulevar Vojvode Stepe 444a, 11060, Belgrade, Serbia aut NATIONALLICENCE 700 1- Radović Svetlana Institute for Genetics and Genetic Engineering, Bulevar Vojvode Stepe 444a, 11060, Belgrade, Serbia aut NATIONALLICENCE 700 1- Maksimović Vesna Institute for Genetics and Genetic Engineering, Bulevar Vojvode Stepe 444a, 11060, Belgrade, Serbia aut NATIONALLICENCE 700 1- Lazarević Jelica Institute for Biological Research "S. Stanković”, University of Belgrade, Despota Stefana 142, 11060, Belgrade, Serbia aut NATIONALLICENCE 700 1- Vinterhalter Branka Institute for Biological Research "S. Stanković”, University of Belgrade, Despota Stefana 142, 11060, Belgrade, Serbia aut NATIONALLICENCE 700 1- Nešković Mirjana Institute for Biological Research "S. Stanković”, University of Belgrade, Despota Stefana 142, 11060, Belgrade, Serbia aut NATIONALLICENCE 700 1- Smigocki Ann Molecular Plant Pathology Laboratory, USDA-ARS, 20705, Beltsville, MD, USA aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 289-294 0167-6857 91:3<289 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer