caa a22 4500 463236645 CHVBK 20180405153259.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s11240-007-9288-2 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9288-2 Effects of colchicine on anther and microspore culture of bread wheat ( Triticum aestivum L.) [Elektronische Daten] [Mercedes Soriano, Luis Cistué, María Vallés, Ana Castillo] The aim of this work was to study the effects of colchicine application on chromosome doubling and androgenic response in anther and microspore culture of different bread wheat genotypes. Colchicine was applied during a mannitol stress pretreatment or during the first 48h of culture at concentrations of 0, 150 and 300mgl−1. When colchicine was applied during stress pretreatment, the percentage of doubling depended on genotype and concentration. A significant increase in doubling was observed with 300mgl−1 in the low androgenic responding cv. Caramba. Colchicine incorporation during the first hours of culture improved percentage of doubling in all genotypes, in both anther and microspore culture. Application of 300mgl−1 colchicine improved the percentage of doubling in the two low responding genotypes, to 118% of control in DH24033, and 75% in Caramba in microspore and anther culture, respectively. Concerning the androgenic response, the effect of colchicine on embryo formation and percentage of green plants depended on the genotype and on the culture method. In cv. Pavon, a 2- and a 3-fold increase in percentage of embryogenesis and green plants, respectively, were obtained with 300mgl−1 colchicine in microspore culture. However, no significant differences in these two variables were observed in anther culture. The number of green doubled haploid (DH) plants reflects the index of success of the procedure. Regardless of the culture method, when colchicine was incorporated during the first hours of culture, the number of green DH plants increased significantly in three of four genotypes. These results confirm the usefulness of colchicine application during the first hours of culture in wheat breeding programs. Springer Science+Business Media B.V., 2007 Doubled haploid nationallicence Chromosome doubling nationallicence Androgenesis nationallicence Poaceae nationallicence Stress pretreatment nationallicence Soriano Mercedes Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas (CSIC), Avda Montañana 1005, 50059, Zaragoza, Spain aut Cistué Luis Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas (CSIC), Avda Montañana 1005, 50059, Zaragoza, Spain aut Vallés María Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas (CSIC), Avda Montañana 1005, 50059, Zaragoza, Spain aut Castillo Ana Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas (CSIC), Avda Montañana 1005, 50059, Zaragoza, Spain aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 225-234 0167-6857 91:3<225 2007 91 11240 https://doi.org/10.1007/s11240-007-9288-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9288-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Soriano Mercedes Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas (CSIC), Avda Montañana 1005, 50059, Zaragoza, Spain aut NATIONALLICENCE 700 1- Cistué Luis Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas (CSIC), Avda Montañana 1005, 50059, Zaragoza, Spain aut NATIONALLICENCE 700 1- Vallés María Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas (CSIC), Avda Montañana 1005, 50059, Zaragoza, Spain aut NATIONALLICENCE 700 1- Castillo Ana Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas (CSIC), Avda Montañana 1005, 50059, Zaragoza, Spain aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 225-234 0167-6857 91:3<225 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer