caa a22 4500 463236653 CHVBK 20180405153259.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s11240-007-9291-7 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9291-7 Asymbiotic germination of ornamental Vanda: invitro germination and development of three hybrids [Elektronische Daten] [T. Johnson, M. Kane] The majority of potted flowering orchids sold in the United States are Phalaenopsis hybrids. However, demand for other genera will undoubtedly increase as consumers become accustomed to growing orchids in the home. The objective of this study was to compare the germination and development of three Vanda hybrids, a genus with mass market potential. Specifically, we evaluated whether hybrids with different pedigrees have significantly different culture requirements. After 12weeks culture, differences in germination and development were found among the three hybrids screened. Generally, seeds of Vanda Motes Primrose×Ascocenda Tavivat (hybrid S014) showed higher germination (82.0-95.3%) than seeds of V.Paki×(V. tessellata×V. cristata; hybrid S005) or (V.Joan Warne×V.Paki)×V. Loke (hybrid S013) when cultured on Knudson C, half-strength Murashige & Skoog, or PhytoTechnology Orchid Seed Sowing Medium under three photoperiods (8/16h, 12/12h, 16/12h; light/dark). Protocorms of S005 and S013 did not develop beyond Stage 3 (first leaf present) under any of the conditions tested, while S014 seeds cultured on PhytoTechnology Orchid Seed Sowing Medium developed to Stage 4 (one leaf and ≥1 roots present; 0.5-0.6%) and 5 (two leaves and ≥1 roots present; 2.7-5.6%) under all photoperiods tested. All hybrids appeared to undergo similar early development (Stages 0-3). Limited advanced development of Hybrids S005 and S013 may be due either to inadequate culture conditions or low vigor of these hybrids. These data indicate that commercial production of some Vanda hybrids may be limited by slow growth and development. Efforts to breed hybrids which germinate and develop rapidly may be required to improve the mass market potential of Vanda hybrids. Springer Science+Business Media B.V., 2007 Orchid nationallicence Seed germination nationallicence Seedling development nationallicence Propagation nationallicence Light microscopy nationallicence Scanning electron microscopy nationallicence ½MS : Half-strength Murashige & Skoog medium nationallicence AC : Activated charcoal nationallicence CPD : Critical point drying nationallicence DD : Distilled deionized nationallicence FAA : Formalin acetic acid nationallicence KC : Knudson C medium nationallicence L/D : Light/dark nationallicence MS : Murashige and Skoog medium nationallicence P723 : PhytoTechnology Orchid Seed Sowing Medium nationallicence SEM : Scanning electron microscopy nationallicence TZ : Tetrazolium nationallicence VW : Vacin and Went medium nationallicence Johnson T. Environmental Horticulture Department, University of Florida, P.O. Box 110675, 32611, Gainesville, FL, USA aut Kane M. Environmental Horticulture Department, University of Florida, P.O. Box 110675, 32611, Gainesville, FL, USA aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 251-261 0167-6857 91:3<251 2007 91 11240 https://doi.org/10.1007/s11240-007-9291-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9291-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Johnson T. Environmental Horticulture Department, University of Florida, P.O. Box 110675, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Kane M. Environmental Horticulture Department, University of Florida, P.O. Box 110675, 32611, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 251-261 0167-6857 91:3<251 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer