caa a22 4500 463236688 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s11240-007-9285-5 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9285-5 Comparative analysis of the differential regeneration response of various genotypes of Triticum aestivum , Triticum durum and Triticum dicoccum [Elektronische Daten] [Harsh Chauhan, Srinivas Desai, Paramjit Khurana] An efficient genotype independent, invitro regeneration system was developed for nine popular Indian wheat cultivars, three each of Triticum aestivum L. viz., CPAN1676, HD2329 and PBW343, Triticum durum Desf. viz., PDW215, PDW233 and WH896, and Triticum dicoccum Schrank. Schubl. viz., DDK1001, DDK1025 and DDK1029, by manipulating the concentration and time of exposure to the growth regulator, thidiazuron (TDZ). A total of 18 (for immature inflorescence and embryo explant) and six (for mature embryo explant) different combinations of growth regulators were tried for callusing and regeneration, respectively. Media combination with low concentration of TDZ (2.2μM) in combination to auxin and/or cytokinin (depending upon culture stage), was found to be effective for immature and mature explants. Compact, nodular and highly embryogenic calli were obtained by using immature embryo, immature inflorescence and mature embryo explants, and regeneration frequency up to 25 shoots/explant with an overall 80% regeneration was achieved. Comparable regeneration frequency was achieved for mature embryo explants. No separate hormone combination for rooting was required and plantlets ready to transfer to soil could be obtained in a short period of 8-10weeks. This protocol can be used for raising transgenic plants for functional genomics analysis of agronomically important traits in the three species of wheat. Springer Science+Business Media B.V., 2007 Callus nationallicence Genotype nationallicence Immature inflorescence nationallicence Immature embryo nationallicence Mature embryo nationallicence Regeneration nationallicence Somatic embryogenesis nationallicence Thidiazuron nationallicence 2,4-D : (2,4-Dichlorophenoxy) acetic acid nationallicence TDZ : 1-Phenyl-3-(1,2,3-Thia-Diazol-5-YL) urea nationallicence DAP : Days after pollination nationallicence CM : Callus media nationallicence RM : Regeneration media nationallicence Inf : Inflorescence nationallicence Chauhan Harsh Department of Plant Molecular Biology, Delhi University South Campus, Benito Juarez Road, Dhaula Kuan, 110 021, New Delhi, India aut Desai Srinivas Department of Plant Molecular Biology, Delhi University South Campus, Benito Juarez Road, Dhaula Kuan, 110 021, New Delhi, India aut Khurana Paramjit Department of Plant Molecular Biology, Delhi University South Campus, Benito Juarez Road, Dhaula Kuan, 110 021, New Delhi, India aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 191-199 0167-6857 91:3<191 2007 91 11240 https://doi.org/10.1007/s11240-007-9285-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9285-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chauhan Harsh Department of Plant Molecular Biology, Delhi University South Campus, Benito Juarez Road, Dhaula Kuan, 110 021, New Delhi, India aut NATIONALLICENCE 700 1- Desai Srinivas Department of Plant Molecular Biology, Delhi University South Campus, Benito Juarez Road, Dhaula Kuan, 110 021, New Delhi, India aut NATIONALLICENCE 700 1- Khurana Paramjit Department of Plant Molecular Biology, Delhi University South Campus, Benito Juarez Road, Dhaula Kuan, 110 021, New Delhi, India aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 191-199 0167-6857 91:3<191 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer