caa a22 4500 463236696 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s11240-007-9286-4 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9286-4 A transgenic tropical maize line generated by the direct transformation of the embryo-scutellum by A. tumefaciens [Elektronische Daten] [Angel Valdez-Ortiz, Sergio Medina-Godoy, M. Valverde, Octavio Paredes-López] An efficient Agrobacterium-mediated transformation system, from which transgenic tropical maize plants were directly generated without previous crosses with laboratory or temperate lines, was established. Experimental evaluations were focused on two main issues: (i) establishment of appropriate tissue culture conditions, which induced somatic embryogenesis from the scutellum-cells, and (ii) the delivery of T-DNA toward these cells. High rates of embryogenic-calli, mainly generated from the embryo-scutellum, were obtained when 15mgl−1 AgNO3 were included into the N6-based induction medium; rates up to 19 plants per gram were regenerated from these induced calli. Regarding the Agrobacterium strains evaluated for their transformation capability on the tropical maize line LPC13 used here, best results were obtained from the EHA105 cells when applied at OD550nm=0.5-1.0. Physical microwounds before the Agro-infection proved to be an excellent way to promoting both the T-DNA transferring toward the embryo-scutellum and the increasing of rates of transient GUS expression. The highest frequencies of transient GUS expression corresponding to the scutellum-cells as well as the regeneration of whole transgenic plants emerged from them, were obtained using immature embryos wounded by bombarding at 80lb/in2 followed for vacuum infiltration before and during the Agro-infection, respectively, or using embryos wounded by 5s-sonication (without vacuum infiltration) before the Agro-infection. Transformation frequencies up to 5.41% and 6.82% were obtained from the Agro-infected embryos wounded by particle-bombardment and sonication, respectively. Analyses of the progenies confirmed the sexual transmission of the introduced genes and their stable expression. Springer Science+Business Media B.V., 2007 Agro -infection nationallicence Embryo-scutellum nationallicence Somatic-embryogenesis nationallicence Silver nitrate nationallicence Wounds nationallicence Particle-bombardment nationallicence Sonication nationallicence Valdez-Ortiz Angel Programa Regional del Noroeste para el Doctorado en Biotecnología, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Sinaloa, Av. de las Américas y Josefa Ortiz (Ciudad Universitaria), 80000, Culiacan, Sinaloa, Mexico aut Medina-Godoy Sergio Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, CIIDIR-IPN, Apdo. Postal 280, 81101, Guasave, Sinaloa, Mexico aut Valverde M. Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Apdo Postal 629, 36821, Irapuato, Guanajuato, Mexico aut Paredes-López Octavio Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Apdo Postal 629, 36821, Irapuato, Guanajuato, Mexico aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 201-214 0167-6857 91:3<201 2007 91 11240 https://doi.org/10.1007/s11240-007-9286-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9286-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Valdez-Ortiz Angel Programa Regional del Noroeste para el Doctorado en Biotecnología, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Sinaloa, Av. de las Américas y Josefa Ortiz (Ciudad Universitaria), 80000, Culiacan, Sinaloa, Mexico aut NATIONALLICENCE 700 1- Medina-Godoy Sergio Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, CIIDIR-IPN, Apdo. Postal 280, 81101, Guasave, Sinaloa, Mexico aut NATIONALLICENCE 700 1- Valverde M. Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Apdo Postal 629, 36821, Irapuato, Guanajuato, Mexico aut NATIONALLICENCE 700 1- Paredes-López Octavio Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Apdo Postal 629, 36821, Irapuato, Guanajuato, Mexico aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 201-214 0167-6857 91:3<201 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer