caa a22 4500 46323670X CHVBK 20180405153300.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s11240-007-9283-7 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9283-7 Radical disinfestation protocol eliminates invitro contamination in Guava ( Psidium guajava L.) seeds [Elektronische Daten] [N. Ali, R. Mulwa, M. Norton, R. Skirvin] Controlling contamination invitro is one of the basic requirements for successful tissue culture technology. In preliminary studies, invitro contamination of guava seeds was almost 100%, even when disinfested with bleach. To overcome this problem, we developed a unique method to control contamination using bleach and strong acids. Submerging guava seeds in 10% HCl for 24-72h followed by a 30min treatment with 10% bleach (NaOCl) reduced contamination rates from 98 to 0%. Substituting 5% H2SO4 for 12h for 10% HCL gave similar results. In addition to eliminating contamination, acid-treated seeds germinated faster invitro than control seeds germinated in a greenhouse (15 vs. 40days, respectively). Springer Science+Business Media B.V., 2007 Tissue culture nationallicence Difficult-to-root nationallicence Tropical fruits nationallicence Scarification nationallicence Ali N. Northwest Frontier Province (NWFP) Agricultural University, Peshawar, Pakistan aut Mulwa R. Crops, Horticulture and Soil Sciences Department, Egerton University, P.O. Box 536, Egerton, Kenya aut Norton M. Edward R. Madigan Laboratory, University of Illinois, 258, 1201 W. Gregory Drive, 61801, Urbana, IL, USA aut Skirvin R. Edward R. Madigan Laboratory, University of Illinois, 258, 1201 W. Gregory Drive, 61801, Urbana, IL, USA aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 295-298 0167-6857 91:3<295 2007 91 11240 https://doi.org/10.1007/s11240-007-9283-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9283-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ali N. Northwest Frontier Province (NWFP) Agricultural University, Peshawar, Pakistan aut NATIONALLICENCE 700 1- Mulwa R. Crops, Horticulture and Soil Sciences Department, Egerton University, P.O. Box 536, Egerton, Kenya aut NATIONALLICENCE 700 1- Norton M. Edward R. Madigan Laboratory, University of Illinois, 258, 1201 W. Gregory Drive, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Skirvin R. Edward R. Madigan Laboratory, University of Illinois, 258, 1201 W. Gregory Drive, 61801, Urbana, IL, USA aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 295-298 0167-6857 91:3<295 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer