caa a22 4500 463236718 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s11240-007-9294-4 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9294-4 Induction, development and maturation of somatic embryos in Bunge's pine ( Pinus bungeana Zucc. ex Endl.) [Elektronische Daten] [Cun-Xu Zhang, Qian Li, Lisheng Kong] Embryogenic tissue was induced from developing immature zygotic embryos in Bunge's pine (Pinus bungeana Zucc. ex Endl.). Induction rate reached 84.4% with our best treatment. Zygotic embryos were dissected from megagametophytes and inoculated on different induction media, DCR1 (Gupta PK, Durzan DJ (1985) Plant Cell Rep 4:177-179), BM1 (Gupta PK, Pullman G (1991) U.S. Patent No. 5,036,00) and MSG (Becwar MR, etal. (1988) Somatic cell genetic of woody plants. Kluwer Academic Publishers, Dordrecht, pp. 1-18), supplemented with 2,4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzylaminopurine (BA). DCR1 was the best medium for initiating embryogenic tissue. Induction rates were affected significantly by developmental stages of explants. The highest induction rate was obtained with embryos collected on either June 20 or June 30 with 10mgl−1 2, 4-D and 4mgl−1 BA. Embryogenic tissue was subcultured monthly on DCR1 medium supplemented with 0.3mgl−1 2, 4-D and 0.2mgl−1 α-naphthaleneacetic acid. In order to enhance embryo maturation, embryogenic tissue was transferred onto DCR1 medium for two weeks, in which 1,000mgl−1 myo-inositol was included and all plant growth regulators were eliminated. This pretreated tissue was then transferred onto a maturation medium that was DCR1 medium containing 50gl−1 sucrose and 0.1mgl−1 indolebutyric acid. In this study, benefits of embryo maturation were not observed when abscisic acid and polyethylene glycol were applied in the culture. Springer Science+Business Media B.V., 2007 Bunge's pine nationallicence Conifer nationallicence Pinus sp nationallicence Somatic embryogenesis nationallicence Zhang Cun-Xu College of Forestry, Northwest A&F University, 712100, Yangling, Shaanxi, P.R. China aut Li Qian Development Center of New technique Application, Ningxia University, 750021, Yinchuan, Ningxia, P.R. China aut Kong Lisheng Centre for Forest Biology, Department of Biology, University of Victoria, 3800 Finnerty Rd, V8W 3N5, Victoria, BC, Canada aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 273-280 0167-6857 91:3<273 2007 91 11240 https://doi.org/10.1007/s11240-007-9294-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9294-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Cun-Xu College of Forestry, Northwest A&F University, 712100, Yangling, Shaanxi, P.R. China aut NATIONALLICENCE 700 1- Li Qian Development Center of New technique Application, Ningxia University, 750021, Yinchuan, Ningxia, P.R. China aut NATIONALLICENCE 700 1- Kong Lisheng Centre for Forest Biology, Department of Biology, University of Victoria, 3800 Finnerty Rd, V8W 3N5, Victoria, BC, Canada aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 273-280 0167-6857 91:3<273 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer