caa a22 4500 463236726 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s11240-007-9295-3 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9295-3 Improving genetic transformation of European chestnut and cryopreservation of transgenic lines [Elektronische Daten] [E. Corredoira, M. San-José, A. Vieitez, A. Ballester] The aim of the present work was to study the effect of the developmental stage of the somatic embryos and of the genotype on the genetic transformation of embryogenic lines of European chestnut (Castanea sativa Mill.) and the cryopreservation of the embryogenic lines that are generated. As an initial source of explants in the transformation experiments, it was found that the use of somatic embryos isolated in the globular stage or clumps of 2-3 embryos in globular/heart-shaped stages was more effective (30%) than when embryos at the cotyledonary stage were used (6.7%). All of the seven genotypes tested were transformed, and transformation efficiency was clearly genotype dependent. Three transgenic lines were successfully cryopreserved using the vitrification procedure, and the stable integration of the uidA gene into the transgenic chestnut plants that were regenerated subsequent to cryopreservation was demonstrated. Springer Science+Business Media B.V., 2007 Agrobacterium nationallicence Castanea sativa nationallicence Embryogenic lines nationallicence BA : 6-benzyladenine nationallicence DMSO : dimethylsulfoxide nationallicence MS : Murashige and Skoog medium nationallicence NAA : α-Naphthaleneacetic acid nationallicence PVS2 : Plant vitrification solution 2 nationallicence LN : Liquid nitrogen nationallicence Corredoira E. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avenida de Vigo, s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut San-José M. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avenida de Vigo, s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut Vieitez A. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avenida de Vigo, s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut Ballester A. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avenida de Vigo, s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 281-288 0167-6857 91:3<281 2007 91 11240 https://doi.org/10.1007/s11240-007-9295-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9295-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Corredoira E. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avenida de Vigo, s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- San-José M. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avenida de Vigo, s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Vieitez A. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avenida de Vigo, s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Ballester A. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avenida de Vigo, s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 281-288 0167-6857 91:3<281 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer