caa a22 4500 463236734 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s11240-007-9289-1 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9289-1 Production of herbicide-resistant transgenic sweet potato plants through Agrobacterium tumefaciens method [Elektronische Daten] [Hye Choi, Thummala Chandrasekhar, Hyo-Yeon Lee, Kyung-Moon Kim] Transgenic herbicide-resistant sweet potato plants [Ipomoea batatas (L.) Lam.] were produced through Agrobacterium-mediated transformation system. Embryogenic calli derived from shoot apical meristems were infected with Agrobacterium tumefaciens strain EHA105 harboring the pCAMBIA3301 vector containing the bar gene encoding phosphinothricin N-acetyltransferase (PAT) and the gusA gene encoding β-glucuronidase (GUS). The PPT-resistant calli and plants were selected with 5 and 2.5mgl−1 PPT, respectively. Soil-grown plants were obtained 28-36weeks after Agrobacterium-mediated transformation. Genetic transformation of the regenerated plants growing under selection was demonstrated by PCR, and Southern blot analysis revealed that one to three copies of the transgene were integrated into the plant genome of each transgenic plant. Expression of the bar gene in transgenic plants was confirmed by RT-PCR and application of herbicide. Transgenic plants sprayed with Basta containing 900mgl−1 of glufosinate ammonium remained green and healthy. The transformation frequency was 2.8% determined by herbicide application which was high when compared to our previous biolistic method. In addition, possible problems with multiple copies of transgene were also discussed. We therefore report here a successful and reliable Agrobacterium-mediated transformation of the bar gene conferring herbicide-resistance and this method may be useful for routine transformation and has the potential to develop new varieties of sweet potato with several important genes for value-added traits such as enhanced tolerance to the herbicide Basta. Springer Science+Business Media B.V., 2007 Agrobacterium tumefaciens nationallicence bar nationallicence Basta nationallicence gusA nationallicence Ipomoea batatas nationallicence Transformation nationallicence AS : Acetosyringone nationallicence BAP : 6-Benzylaminopurine nationallicence CPM : Callus-proliferating medium nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence GUS : β-Glucuronidase nationallicence MES : 2-(N-morpholino) ethanesulfonic acid nationallicence MS : Murashige and Skoog (1962) nationallicence PAT : Phosphinothricin N-acetyltransferase nationallicence PPT : Phosphinothricin nationallicence RT-PCR : Reverse transcriptase-PCR nationallicence YEP : Yeast extract and peptone nationallicence Choi Hye Kumho Life and Environmental Science Laboratory, Chonnam National University, 1 Oryong-dong, Buk-gu, 500-712, Gwangju, Republic of Korea aut Chandrasekhar Thummala Faculty of Biotechnology, Cheju National University, 1 Ara-dong, 690-756, Jeju, Republic of Korea aut Lee Hyo-Yeon Faculty of Biotechnology, Cheju National University, 1 Ara-dong, 690-756, Jeju, Republic of Korea aut Kim Kyung-Moon Kumho Life and Environmental Science Laboratory, Chonnam National University, 1 Oryong-dong, Buk-gu, 500-712, Gwangju, Republic of Korea aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 235-242 0167-6857 91:3<235 2007 91 11240 https://doi.org/10.1007/s11240-007-9289-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9289-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choi Hye Kumho Life and Environmental Science Laboratory, Chonnam National University, 1 Oryong-dong, Buk-gu, 500-712, Gwangju, Republic of Korea aut NATIONALLICENCE 700 1- Chandrasekhar Thummala Faculty of Biotechnology, Cheju National University, 1 Ara-dong, 690-756, Jeju, Republic of Korea aut NATIONALLICENCE 700 1- Lee Hyo-Yeon Faculty of Biotechnology, Cheju National University, 1 Ara-dong, 690-756, Jeju, Republic of Korea aut NATIONALLICENCE 700 1- Kim Kyung-Moon Kumho Life and Environmental Science Laboratory, Chonnam National University, 1 Oryong-dong, Buk-gu, 500-712, Gwangju, Republic of Korea aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/3(2007-12-01), 235-242 0167-6857 91:3<235 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer