caa a22 4500 463236750 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s11240-007-9280-x doi (NATIONALLICENCE)springer-10.1007/s11240-007-9280-x In vitro propagation of Helleborus species [Elektronische Daten] [Emmy Dhooghe, Marie-Christine Van Labeke] A general in vitro cloning system was established for four Helleborus species: H. argutifolius, H. foetidus, H. niger and H. orientalis. The plant material was introduced in vitro from axillary buds. A Murashige and Skoog (MS)—based medium (Murashige and Skoog 1962) was used supplemented with 2% (w/v) sucrose, 2-isopentenyladenine (2-iP) and 6-benzylaminopurine (BA). Multiplication rates depended on the genotype and varied from 1.3 for H. foetidus till 3.8 for H. niger. The first results showed that the rooting phase could be done ex vitro. Rooting was induced by a drench for one week in a solution of indole-3-butyric acid (IBA -3mgl−1) and 1-naphthaleneacetic acid (NAA-1mgl−1) at 5°C. Springer Science+Business Media B.V., 2007 Argutifolius nationallicence Axillary bud nationallicence Foetidus nationallicence Niger nationallicence Orientalis nationallicence Ranunculaceae nationallicence BA : 6-benzylaminopurine nationallicence IBA : Indole-3-butyric acid nationallicence 2-iP : 2-isopentenyladenine nationallicence MS : Murashige and Skoog salts nationallicence NAA : 1-naphtaleneacetic acid nationallicence NOA : Naphthoxyacetic acid nationallicence PPM™ : Preservative for Plant Tissue Culture Media nationallicence Dhooghe Emmy Department of Plant Production, Genth University, Coupure links 653, 9000, Gent, Belgium aut Van Labeke Marie-Christine Department of Plant Production, Genth University, Coupure links 653, 9000, Gent, Belgium aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 175-177 0167-6857 91:2<175 2007 91 11240 https://doi.org/10.1007/s11240-007-9280-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9280-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dhooghe Emmy Department of Plant Production, Genth University, Coupure links 653, 9000, Gent, Belgium aut NATIONALLICENCE 700 1- Van Labeke Marie-Christine Department of Plant Production, Genth University, Coupure links 653, 9000, Gent, Belgium aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 175-177 0167-6857 91:2<175 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer