caa a22 4500 463236785 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s11240-007-9281-9 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9281-9 Production of doubled haploids in durum wheat ( Triticum durum Desf.) through culture of unpollinated ovaries [Elektronische Daten] [Olfa Slama-Ayed, Hajer Slim-Amara] The objective of this work was to produce doubled haploid plants from durum wheat through gynogenesis using unpollinated ovary culture of three local Tunisian genotypes (Jenah Khotifa, Hmira, Azizi) and three improved cultivars (Karim, Khiar, Razzek). A total of 12,000 unpollinated ovaries were cultured in this study. Spikes were either pretreated at 4°C for 14days or at 4°C in a mannitol solution (0.3M) for 7days. Induction was performed using two media. We showed that ovary development, callus and plantlet regeneration was influenced significantly by genotype and growth conditions. The highest regeneration frequency was obtained when the microspore population was in the late mononucleate to binucleate stage. Our results suggested that the cold pretreatment for 14days was more efficient than the cold treatment in a mannitol solution. Furthermore, the addition of 2,4-D, vitamins and glutamine, and the use of maltose as sugar source in media improved the ovary culture. When the unpollinated ovaries were cultured under the conditions found to be optimal in the present study, a total of 84 plants were produced, all green and haploid. The best levels for regenerated plants were obtained with the cultivars Khiar (3.5%), Hmira (3.1%) and Karim (1.5%). Fertile doubled haploid plants were obtained by colchicine treatment. This result represents a modern tool for breeders to produce durum wheat homozygous lines in a few months. Springer Science+Business Media B.V., 2007 In vitro gynogenesis nationallicence Doubled haploid nationallicence Durum wheat nationallicence Pretreatment nationallicence DH : Doubled haploid plant nationallicence 2,4-D : 2,4 Dichlorophenoxyacetic acid nationallicence 2ip : 6-(γ, γ-Dimethylamino)purine nationallicence NAA : Naphtaleneacetic acid nationallicence Slama-Ayed Olfa Laboratory of Genetic and Plant Breeding, Department of Agronomy and Plant Biotechnology, National Agronomic Institute of Tunisia, 43, Avenue Charles Nicolle 1082, Tunis, Mahrajene, Tunisia aut Slim-Amara Hajer Laboratory of Genetic and Plant Breeding, Department of Agronomy and Plant Biotechnology, National Agronomic Institute of Tunisia, 43, Avenue Charles Nicolle 1082, Tunis, Mahrajene, Tunisia aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 125-133 0167-6857 91:2<125 2007 91 11240 https://doi.org/10.1007/s11240-007-9281-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9281-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Slama-Ayed Olfa Laboratory of Genetic and Plant Breeding, Department of Agronomy and Plant Biotechnology, National Agronomic Institute of Tunisia, 43, Avenue Charles Nicolle 1082, Tunis, Mahrajene, Tunisia aut NATIONALLICENCE 700 1- Slim-Amara Hajer Laboratory of Genetic and Plant Breeding, Department of Agronomy and Plant Biotechnology, National Agronomic Institute of Tunisia, 43, Avenue Charles Nicolle 1082, Tunis, Mahrajene, Tunisia aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 125-133 0167-6857 91:2<125 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer