caa a22 4500 463236807 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s11240-007-9238-z doi (NATIONALLICENCE)springer-10.1007/s11240-007-9238-z Axillary proliferation and tuberisation of Dioscorea cayenensis-D. rotundata complex [Elektronische Daten] [Paul Ondo Ovono, Claire Kevers, Jacques Dommes] Yams (Dioscorea spp) are tuber crops used as staple food in Africa because of their nutritional value. However agronomic constraints, phytosanitary problems and the lack of good healthy planting material restrict their production. In contrast to the inefficiency of traditional method of planting, tissue culture techniques allow to increase the multiplication and the rapid production of pathogen- free plant material. This work was undertaken to provide farmers in African countries with healthy microplants and microtubers as seeds. In vitro nodal segments of two varieties of local yams D. cayenensis-D. rotundata complex (cv. ‘Singo', cv. ‘Singou' and cv. ‘Gnidou') were micropropagated on the modified medium of Murashige and Skoog. The morphogenesis, the growth of microplants and microtuber formation have been found to be controlled by external factors that act individually and synergistically. Addition of kinetin (2mgl−1) to the culture media could reduce multiplication rate (node number) of some clones. An increase of the sucrose concentration from 3% to 5% induced no change in the multiplication and tuberisation parameters. An important reduction of the multiplication (shoot number, height and node number) and the tuberisation (tuber number and length) was observed with 8% sucrose. Multiplication (shoot and node number) was increased in the presence of jasmonic acid (10μM). JA also induced an increase of tuber number in the absence of Kin. Multiplication of yam by in vitro growth of nodal segments is a way for rapid clonal multiplication and could allow solving the problem of lack of seed material faced by farmers. This method could also be used for multiplication of elite cultivars, independently of the growing season. Springer Science+Business Media B.V., 2007 Cytokinin nationallicence Dioscorea cayenensis-D. rotundata nationallicence Jasmonic acid nationallicence Micropropagation nationallicence Sucrose nationallicence Yam nationallicence JA : Jasmonic acid nationallicence Kin : Kinetin nationallicence MS : Murashige and Skoog nationallicence Ondo Ovono Paul Plant Molecular Biology and Biotechnology Unit, University of Liege, Sart Tilman, B-22, 4000, Liege, Belgium aut Kevers Claire Plant Molecular Biology and Biotechnology Unit, University of Liege, Sart Tilman, B-22, 4000, Liege, Belgium aut Dommes Jacques Plant Molecular Biology and Biotechnology Unit, University of Liege, Sart Tilman, B-22, 4000, Liege, Belgium aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 107-114 0167-6857 91:2<107 2007 91 11240 https://doi.org/10.1007/s11240-007-9238-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9238-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ondo Ovono Paul Plant Molecular Biology and Biotechnology Unit, University of Liege, Sart Tilman, B-22, 4000, Liege, Belgium aut NATIONALLICENCE 700 1- Kevers Claire Plant Molecular Biology and Biotechnology Unit, University of Liege, Sart Tilman, B-22, 4000, Liege, Belgium aut NATIONALLICENCE 700 1- Dommes Jacques Plant Molecular Biology and Biotechnology Unit, University of Liege, Sart Tilman, B-22, 4000, Liege, Belgium aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 107-114 0167-6857 91:2<107 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer