caa a22 4500 463236815 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s11240-007-9236-1 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9236-1 Somatic embryogenesis from mature Bambusa balcooa Roxburgh as basis for mass production of elite forestry bamboos [Elektronische Daten] [Koen Gillis, Johan Gielis, Hilde Peeters, Emmy Dhooghe, Jan Oprins] A reliable protocol for mass propagation via somatic embryogenesis in mature bamboos has been established using pseudospikelets of Bambusa balcooa. Fourty percent of the explants gave rise to multiple regenerants within 4months. This conversion rate is sufficiently high to use the process in commercial mass production. Further, shoot apical meristems can also be used as primary explants without lost of efficiency. Regenerated plants were uniform and identical to the mother plant and to plants obtained by axillary branching with respect to growth characteristics and morphology. Furthermore, epigenetic changes could not be detected by Methylation Sensitive AFLP (MSAP). During the complete process no changes in ploidy level could be observed. The process allows for a cost reduction for this tropical bamboo for forestry of up to 57% compared to micropropagation via axillary branching. For the first time, a reliable process based on somatic embryogenesis has been developed that is well suited for commercial micropropagation of elite mature bamboos. Springer Science+Business Media B.V., 2007 Bamboo nationallicence Commercial micropropagation nationallicence In vitro nationallicence Monocot nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence AFLP : Amplified Fragment Length Polymorphism nationallicence BAP : 6-Benzylaminopurine nationallicence DAPI : 6′-Diamidino-2 phenylindole nationallicence FAA : Formalin, acetic acid, 70% ethanol 5: 5: 90 nationallicence FESEM : Field emission scanning electron microscopy nationallicence MS : Murashige and Skoog medium (Murashige and Skoog 1962) nationallicence MSAP : Methylation Sensitive AFLP nationallicence PAR : Photosynthetic active radiation nationallicence SE : Somatic embryogenesis nationallicence Gillis Koen Oprins Plant NV, St.-Lenaartsesteenweg 91, 2310, Rijkevorsel, Belgium aut Gielis Johan Oprins Plant NV, St.-Lenaartsesteenweg 91, 2310, Rijkevorsel, Belgium aut Peeters Hilde Oprins Plant NV, St.-Lenaartsesteenweg 91, 2310, Rijkevorsel, Belgium aut Dhooghe Emmy Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000, Gent, Belgium aut Oprins Jan Oprins Plant NV, St.-Lenaartsesteenweg 91, 2310, Rijkevorsel, Belgium aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 115-123 0167-6857 91:2<115 2007 91 11240 https://doi.org/10.1007/s11240-007-9236-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9236-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gillis Koen Oprins Plant NV, St.-Lenaartsesteenweg 91, 2310, Rijkevorsel, Belgium aut NATIONALLICENCE 700 1- Gielis Johan Oprins Plant NV, St.-Lenaartsesteenweg 91, 2310, Rijkevorsel, Belgium aut NATIONALLICENCE 700 1- Peeters Hilde Oprins Plant NV, St.-Lenaartsesteenweg 91, 2310, Rijkevorsel, Belgium aut NATIONALLICENCE 700 1- Dhooghe Emmy Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000, Gent, Belgium aut NATIONALLICENCE 700 1- Oprins Jan Oprins Plant NV, St.-Lenaartsesteenweg 91, 2310, Rijkevorsel, Belgium aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 115-123 0167-6857 91:2<115 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer