caa a22 4500 463236823 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s11240-007-9282-8 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9282-8 Chlorophyll fluorescence imaging for disease-resistance screening of sugar beet [Elektronische Daten] [Laury Chaerle, Dik Hagenbeek, Erik De Bruyne, Dominique Van Der Straeten] Both biotic and abiotic stresses cause considerable crop yield losses worldwide (Chrispeels, Sadava Plants, genes, and crop biotechnology 2003; Oerke, Dehne Crop Prot 23:275-285 2004). To speed up screening assays in stress resistance breeding, non-contact techniques such as chlorophyll fluorescence imaging can be advantageously used in the quantification of stress-inflicted damage. In comparison with visual spectrum images, chlorophyll fluorescence imaging reveals cell death with higher contrast and at earlier time-points. This technique has the potential to automatically quantify stress-inflicted damage during screening applications. From a physiological viewpoint, screening stress-responses using attached plant leaves is the ideal approach. However, leaf growth and circadian movements interfere with time-lapse monitoring of leaves, making it necessary to fix the leaves to be studied. From this viewpoint, a method to visualise the evolution of chlorophyll fluorescence from excised leaf pieces kept in closed petri dishes offers clear advantages. In this study, the plant-fungus interaction sugar beet-Cercospora beticola was assessed both in attached leaf and excised leaf strip assays. The attached leaf assay proved to be superior in revealing early, pre-visual symptoms and to better discriminate between the lines with different susceptibility to Cercospora. Springer Science+Business Media B.V., 2007 Cercospora beticola Sacc nationallicence Chlorophyll fluorescence imaging nationallicence Plant disease resistance quantification nationallicence Plant-pathogen interaction nationallicence Sugar beet nationallicence Thermography nationallicence BA : 6-Benzylaminopurine nationallicence Chl-FI : Chlorophyll fluorescence imaging nationallicence dpi : Days postinfection nationallicence FL : Chlorophyll fluorescence image captured after low intensity excitation nationallicence FH : Chlorophyll fluorescence image captured after high intensity excitation nationallicence FIS : Fluorescence imaging system nationallicence Chaerle Laury Unit Plant Hormone Signalling and Bio-imaging, Ghent University, K.L. Ledeganckstraat 35, 9000, Gent, Belgium aut Hagenbeek Dik Unit Plant Hormone Signalling and Bio-imaging, Ghent University, K.L. Ledeganckstraat 35, 9000, Gent, Belgium aut De Bruyne Erik Phytopathology Department, SESVanderHave, Soldatenplein Z2, Industriepark 15, 3300, Tienen, Belgium aut Van Der Straeten Dominique Unit Plant Hormone Signalling and Bio-imaging, Ghent University, K.L. Ledeganckstraat 35, 9000, Gent, Belgium aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 97-106 0167-6857 91:2<97 2007 91 11240 https://doi.org/10.1007/s11240-007-9282-8 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9282-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chaerle Laury Unit Plant Hormone Signalling and Bio-imaging, Ghent University, K.L. Ledeganckstraat 35, 9000, Gent, Belgium aut NATIONALLICENCE 700 1- Hagenbeek Dik Unit Plant Hormone Signalling and Bio-imaging, Ghent University, K.L. Ledeganckstraat 35, 9000, Gent, Belgium aut NATIONALLICENCE 700 1- De Bruyne Erik Phytopathology Department, SESVanderHave, Soldatenplein Z2, Industriepark 15, 3300, Tienen, Belgium aut NATIONALLICENCE 700 1- Van Der Straeten Dominique Unit Plant Hormone Signalling and Bio-imaging, Ghent University, K.L. Ledeganckstraat 35, 9000, Gent, Belgium aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 97-106 0167-6857 91:2<97 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer