caa a22 4500 463236831 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s11240-007-9257-9 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9257-9 Evaluation of UV damage at DNA level in Nicotiana plumbaginifolia protoplasts using single cell gel electrophoresis [Elektronische Daten] [Yulheri Abas, Nadia Touil, Micheline Kirsch-Volders, Geert Angenon, Michel Jacobs, Ivan Famelaer] The aim of the present study was to observe the induction and repair of single strand breaks (Ssbs) and double strand breaks (Dsbs) in mesophyll protoplasts of Nicotiana plumbaginifolia, irradiated with UV-C and cultured under light or dark conditions. DNA damage and repair was determined by the neutral and alkaline comet assay to reveal Dsbs and Ssbs respectively. Subculturing protoplasts for 4h at low temperature was essential to reduce the amount of Dsbs to the detection limit of the assay procedure. Light-cultured protoplasts showed a significant increase of Ssbs and Dsbs compared to dark cultured protoplasts, in which the number of Ssbs and Dsbs remained very constant throughout the experiments. UV treatment significantly enhanced the levels of Ssbs and Dsbs in light and dark cultured protoplasts. On average, equal levels of DNA damage were observed under light or dark conditions. Formulations introduced to evaluate the contribution of UV-C or light treatment in repair kinetics of DNA damage, showed that the number of Ssbs, but not of Dsbs, evolved differently for light and dark cultured protoplasts. DNA repair was more rapidly observed under light conditions and occurred in different repair phases. Observations are discussed in relation to the involvement of chromatin remodelling, photosynthetic active radiation and DNA repair mechanisms. Springer Science+Business Media B.V., 2007 Comet assay nationallicence DNA damage and repair nationallicence Plant protoplast nationallicence UV-C irradiation nationallicence Abas Yulheri Laboratory of Plant Genetics, Department of Applied Biological Sciences, Institute of Molecular Biology and Biotechnology, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium aut Touil Nadia Laboratory of Cell Genetics, Department of Biological Sciences, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium aut Kirsch-Volders Micheline Laboratory of Cell Genetics, Department of Biological Sciences, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium aut Angenon Geert Laboratory of Plant Genetics, Department of Applied Biological Sciences, Institute of Molecular Biology and Biotechnology, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium aut Jacobs Michel Laboratory of Plant Genetics, Department of Applied Biological Sciences, Institute of Molecular Biology and Biotechnology, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium aut Famelaer Ivan Laboratory of Plant Genetics, Department of Applied Biological Sciences, Institute of Molecular Biology and Biotechnology, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 145-154 0167-6857 91:2<145 2007 91 11240 https://doi.org/10.1007/s11240-007-9257-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9257-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abas Yulheri Laboratory of Plant Genetics, Department of Applied Biological Sciences, Institute of Molecular Biology and Biotechnology, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium aut NATIONALLICENCE 700 1- Touil Nadia Laboratory of Cell Genetics, Department of Biological Sciences, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium aut NATIONALLICENCE 700 1- Kirsch-Volders Micheline Laboratory of Cell Genetics, Department of Biological Sciences, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium aut NATIONALLICENCE 700 1- Angenon Geert Laboratory of Plant Genetics, Department of Applied Biological Sciences, Institute of Molecular Biology and Biotechnology, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium aut NATIONALLICENCE 700 1- Jacobs Michel Laboratory of Plant Genetics, Department of Applied Biological Sciences, Institute of Molecular Biology and Biotechnology, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium aut NATIONALLICENCE 700 1- Famelaer Ivan Laboratory of Plant Genetics, Department of Applied Biological Sciences, Institute of Molecular Biology and Biotechnology, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 145-154 0167-6857 91:2<145 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer