caa a22 4500 463236866 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s11240-007-9226-3 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9226-3 Effect of enzyme concentrations on protoplast isolation and protoplast culture of Spathiphyllum and Anthurium [Elektronische Daten] [Barbara Duquenne, Tom Eeckhaut, Stefaan Werbrouck, Johan Van Huylenbroeck] Vital protoplasts from Spathiphyllum wallisii ‘Alain' and Anthurium scherzerianum ‘238' were isolated from both somatic embryos and leaves. The highest yields were obtained when 1.5% cellulase, 0.5% macerase and 0.5% driselase were used for Spathiphyllum wallisii leaves and 0.5% cellulase, 0.3% macerase and 0.5% driselase for Anthurium scherzerianum embryos. About 1×106 protoplasts g−1 and 1×105 protoplasts g−1 could be isolated from leaves and embryos, respectively. For protoplast fusion Spathiphyllum wallisii ‘Alain' and Anthurium scherzerianum ‘238' were mixed in a 1:1 ratio in a fusion solution containing 1mM CaCl2·2H2O, 1mM MES and 0.5M mannitol. Fusion was performed by protoplast alignment under 500Vcm−1 alternating current for 60s and subsequent generation of two pulses of 4500Vcm−1 direct current during 50μs. Development until colony stage was achieved using agarose beads for protoplast culture. Springer Science+Business Media B.V., 2007 Araceae nationallicence Cellulase nationallicence Driselase nationallicence Electrofusion nationallicence Macerase nationallicence Agarose beads nationallicence Somatic embryos nationallicence AC : Alternating current nationallicence DC : Direct current nationallicence FDA : Fluorescein diacetate nationallicence FW : Fresh weight nationallicence KM : Kao and Michayluk nationallicence MES : Morpholino-ethane sulfonic acid nationallicence PP : Protoplasts nationallicence Duquenne Barbara Unit Plant - Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut Eeckhaut Tom Unit Plant - Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut Werbrouck Stefaan Department of Biotechnology, Landscape Architecture and Agriculture, Hogeschool Gent, Voskenslaan 270, 9000, Gent, Belgium aut Van Huylenbroeck Johan Unit Plant - Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 165-173 0167-6857 91:2<165 2007 91 11240 https://doi.org/10.1007/s11240-007-9226-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9226-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Duquenne Barbara Unit Plant - Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut NATIONALLICENCE 700 1- Eeckhaut Tom Unit Plant - Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut NATIONALLICENCE 700 1- Werbrouck Stefaan Department of Biotechnology, Landscape Architecture and Agriculture, Hogeschool Gent, Voskenslaan 270, 9000, Gent, Belgium aut NATIONALLICENCE 700 1- Van Huylenbroeck Johan Unit Plant - Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 91/2(2007-11-01), 165-173 0167-6857 91:2<165 2007 91 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer