caa a22 4500 463236882 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11259-006-3364-7 doi (NATIONALLICENCE)springer-10.1007/s11259-006-3364-7 Recombinant Antigen-based Latex Agglutination Test for Rapid Serodiagnosis of Leptospirosis [Elektronische Daten] [S. Dey, C. Madhan Mohan, P. Ramadass, K. Nachimuthu] A rapid recombinant antigen-based latex agglutination test (LAT) has been developed to detect specific anti-leptospiral antibodies from human and dog sera. The recombinant LipL32 antigen developed and used for detecting the antibodies is specific in detection of the pathogenic serovars of Leptospira as the expression of the LipL32 antigen is restricted only to the pathogenic leptospires. The sensitized latex beads are stable and could be stored at 4°C for more than three months without showing loss of activity for both weakly and strongly positive samples. The test is found to be sensitive, specific and accurate as compared to the standard microscopic agglutination test (MAT). Moreover, the recombinant antigen-coated latex beads could detect the specific anti-leptospiral antibodies in the acute phase of the illness. The test is simple and inexpensive, and is rapid in the management of large numbers of patients. Springer Science + Business Media, Inc., 2007 leptospirosis nationallicence pathogenic nationallicence recombinant LipL32 antigen nationallicence latex agglutination test nationallicence diagnosis nationallicence LAT : latex agglutination test nationallicence MAT : microscopic agglutination test nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence Dey S. Department of Animal Biotechnology, Madras Veterinary College, Vepery, 600 007, Chennai aut Madhan Mohan C. Department of Animal Biotechnology, Madras Veterinary College, Vepery, 600 007, Chennai aut Ramadass P. Department of Animal Biotechnology, Madras Veterinary College, Vepery, 600 007, Chennai aut Nachimuthu K. Department of Animal Biotechnology, Madras Veterinary College, Vepery, 600 007, Chennai aut Veterinary Research Communications Kluwer Academic Publishers 31/1(2007-01-01), 9-15 0165-7380 31:1<9 2007 31 11259 https://doi.org/10.1007/s11259-006-3364-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-3364-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dey S. Department of Animal Biotechnology, Madras Veterinary College, Vepery, 600 007, Chennai aut NATIONALLICENCE 700 1- Madhan Mohan C. Department of Animal Biotechnology, Madras Veterinary College, Vepery, 600 007, Chennai aut NATIONALLICENCE 700 1- Ramadass P. Department of Animal Biotechnology, Madras Veterinary College, Vepery, 600 007, Chennai aut NATIONALLICENCE 700 1- Nachimuthu K. Department of Animal Biotechnology, Madras Veterinary College, Vepery, 600 007, Chennai aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/1(2007-01-01), 9-15 0165-7380 31:1<9 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer