caa a22 4500 463236890 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11259-006-3423-0 doi (NATIONALLICENCE)springer-10.1007/s11259-006-3423-0 Measurement of Carbonic Anhydrase Isozyme VI (CA-VI) in Bovine Sera, Saliva, Milk and Tissues [Elektronische Daten] [T. Nishita, Y. Tanaka, Y. Wada, M. Murakami, T. Kasuya, N. Ichihara, K. Matsui, M. Asari] Concentrations of bovine carbonic anhydrase isozyme VI (CA-IV) in bovine serum, saliva, normal milk, colostrum, submandibular gland, liver, and mammary gland were determined. CA-VI was purified from bovine saliva and an antibody to CA-VI was generated. The concentrations of CA-VI in the saliva (7.8 ± 7.9 μg/ml), serum (2.1± 5.7 ng/ml), milk (7.9 ± 12.1 ng/ml), submandibular gland (284.7 μg/g protein), liver (921.0 ± 180.7 ng/g protein) and mammary gland (399.6 ± 191.2 ng/g protein) were determined by ELISA. No seasonal change in CA-VI levels was observed in normal milk. The concentration of CA-VI in colostrum (day 1 post partum) was 119 ng/ml and decreased rapidly by 1 month following birth. Mammary gland contained much smaller amounts than the submandibular gland. CA-VI mRNA was detected in the liver and mammary gland of cow by RT-PCR. The ELISA used in this study proved to be a precise and sensitive method for determining CA-VI concentrations in saliva, serum, milk and tissue specimens from cows. The ELISA may enable the study of changes in CA-VI associated with hereditary or metabolic disorders of the salivary gland, mammary gland and liver using small samples of saliva, serum or milk. Springer Science + Business Media, Inc., 2007 carbonic anhydrase nationallicence CA-VI nationallicence liver nationallicence mammary gland nationallicence milk nationallicence RT-PCR nationallicence saliva nationallicence serum nationallicence ABC reagent : avidin and biotinylated horseradish peroxidase complex nationallicence CA-VI : secretory carbonic anhydrase isozyme VI nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence PBS : phosphate-buffered saline solution nationallicence BSA : bovine serum albumin nationallicence RT-PCR : reverse transcription-polymerase chain reaction nationallicence Nishita T. Laboratory of Veterinary Physiology 1, School of Veterinary Medicine, Azabu University, Kanagawa aut Tanaka Y. Livestock Industry Research Institute, Kanagawa Prefectural Government, Kanagawa, Japan aut Wada Y. Veterinary Intermedicine 3, School of Veterinary Medicine, Azabu University, Kanagawa aut Murakami M. Molecular Biology, School of Veterinary Medicine, Azabu University, Kanagawa aut Kasuya T. Laboratory of Veterinary Physiology 1, School of Veterinary Medicine, Azabu University, Kanagawa aut Ichihara N. Veterinary Anatomy 1, School of Veterinary Medicine, Azabu University, Kanagawa aut Matsui K. Laboratory of Veterinary Physiology 1, School of Veterinary Medicine, Azabu University, Kanagawa aut Asari M. Veterinary Anatomy 1, School of Veterinary Medicine, Azabu University, Kanagawa aut Veterinary Research Communications Kluwer Academic Publishers 31/1(2007-01-01), 83-92 0165-7380 31:1<83 2007 31 11259 https://doi.org/10.1007/s11259-006-3423-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-3423-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nishita T. Laboratory of Veterinary Physiology 1, School of Veterinary Medicine, Azabu University, Kanagawa aut NATIONALLICENCE 700 1- Tanaka Y. Livestock Industry Research Institute, Kanagawa Prefectural Government, Kanagawa, Japan aut NATIONALLICENCE 700 1- Wada Y. Veterinary Intermedicine 3, School of Veterinary Medicine, Azabu University, Kanagawa aut NATIONALLICENCE 700 1- Murakami M. Molecular Biology, School of Veterinary Medicine, Azabu University, Kanagawa aut NATIONALLICENCE 700 1- Kasuya T. Laboratory of Veterinary Physiology 1, School of Veterinary Medicine, Azabu University, Kanagawa aut NATIONALLICENCE 700 1- Ichihara N. Veterinary Anatomy 1, School of Veterinary Medicine, Azabu University, Kanagawa aut NATIONALLICENCE 700 1- Matsui K. Laboratory of Veterinary Physiology 1, School of Veterinary Medicine, Azabu University, Kanagawa aut NATIONALLICENCE 700 1- Asari M. Veterinary Anatomy 1, School of Veterinary Medicine, Azabu University, Kanagawa aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/1(2007-01-01), 83-92 0165-7380 31:1<83 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer