caa a22 4500 463236955 CHVBK 20180405153300.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11259-006-3290-8 doi (NATIONALLICENCE)springer-10.1007/s11259-006-3290-8 Deduced Amino Acid Sequences Surrounding the Fusion Glycoprotein Cleavage Site and of the Carboxyl-terminus of Haemagglutinin-Neuraminidase Protein of the Avirulent Thermostable Vaccine Strain I-2 of Newcastle disease virus [Elektronische Daten] [P. Wambura, J. Meers, J. Kattenbelt, A. Gould, P. Spradbrow] A single-tube RT-PCR technique generated a 387 bp or 300 bp cDNA amplicon covering the F0 cleavage site or the carboxyl (C)-terminus of the HN gene, respectively, of Newcastle disease virus (NDV) strain I-2. Sequence analysis was used to deduce the amino acid sequences of the cleavage site of F protein and the C-terminus of HN protein, which were then compared with sequences for other NDV strains. The cleavage site of NDV strain I-2 had a sequence motif of 112 RKQGRLIG119, consistent with an avirulent phenotype. Nucleotide sequencing and deduction of amino acids at the C-terminus of HN revealed that strain I-2 had a 7-amino-acid extension (VEILKDGVREARSSR. This differs from the virulent viruses that caused outbreaks of Newcastle disease in Australia in the 1930s and 1990s, which have HN extensions of 0 and 9 amino acids, respectively. Amino acid sequence analyses of the F and HN genes of strain I-2 confirmed its avirulent nature and its Australian origin. Springer Science + Business Media, Inc., 2007 carboxyl-terminus nationallicence chickens nationallicence cleavage site nationallicence fusion protein nationallicence haemagglutinin-neuraminidase nationallicence Newcastle disease nationallicence strain I-2 nationallicence thermostable vaccine nationallicence bp : base pairs nationallicence cDNA : complementary deoxyribose nucleic acid nationallicence F0 protein : fusion uncleaved protein nationallicence Wambura P. School of Veterinary Science, University of Queensland, Brisbane aut Meers J. School of Veterinary Science, University of Queensland, Brisbane aut Kattenbelt J. Clinical Medical Virology Centre, University of Queensland & Sir Albert Sakzewski Virus Research Centre, Herston, Australia aut Gould A. Clinical Medical Virology Centre, University of Queensland & Sir Albert Sakzewski Virus Research Centre, Herston, Australia aut Spradbrow P. School of Veterinary Science, University of Queensland, Brisbane aut Veterinary Research Communications Kluwer Academic Publishers 31/1(2007-01-01), 105-112 0165-7380 31:1<105 2007 31 11259 https://doi.org/10.1007/s11259-006-3290-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-3290-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wambura P. School of Veterinary Science, University of Queensland, Brisbane aut NATIONALLICENCE 700 1- Meers J. School of Veterinary Science, University of Queensland, Brisbane aut NATIONALLICENCE 700 1- Kattenbelt J. Clinical Medical Virology Centre, University of Queensland & Sir Albert Sakzewski Virus Research Centre, Herston, Australia aut NATIONALLICENCE 700 1- Gould A. Clinical Medical Virology Centre, University of Queensland & Sir Albert Sakzewski Virus Research Centre, Herston, Australia aut NATIONALLICENCE 700 1- Spradbrow P. School of Veterinary Science, University of Queensland, Brisbane aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/1(2007-01-01), 105-112 0165-7380 31:1<105 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer