caa a22 4500 463236998 CHVBK 20180405153301.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11259-006-3397-y doi (NATIONALLICENCE)springer-10.1007/s11259-006-3397-y Validation of a Commercially Available Human Immunoturbidimetric Assay for Haptoglobin Determination in Canine Serum Samples [Elektronische Daten] [F. Tecles, S. Subiela, G. Petrucci, C. Panizo, J. Cerón] Haptoglobin is a positive acute-phase protein with a valuable role as a marker of inflammation in both human and veterinary medicine. The aim of this study was to validate a commercially available immunoturbidimetric method designed for human haptoglobin determination (Izasa SA, Barcelona, Spain) for its use in canine samples. Cross-reactivity between anti-human haptoglobin antiserum and canine haptoglobin was found when agarose gel immunodiffusion and ELISA tests were performed. The use of canine pooled serum with haptoglobin concentration of 6.3 g/L as standard provided higher analytical range than commercially available standards. Intra-assay and inter-assay coefficients of variation were 2.49% and 4.60%, respectively. A linear regression model between immunoturbidimetric results and a previously validated spectrophotometric method (Tridelta Development Limited, Ireland) yielded a slope at 95% confidence interval of 0.94 (0.86, 1.02) and y-intercept at 95% confidence interval of 0.11 (−0.59, 0.82). No significant differences were produced by anticoagulants, lipaemia and bilirubinaemia, although haemolysis significantly decreased haptoglobin. A significant increase of haptoglobin concentration was detected in inflammatory conditions such as pyometra and leishmaniasis, in neoplastic conditions, and after glucocorticoid administration. Canine serum haptoglobin concentration can be reliably measured using the commercially available Izasa immunoturbidimetric method developed for human haptoglobin determination. This method is precise and accurate, provides a wider analytical range than previous reported methods, and can be easily automated and used for routine haptoglobin determination in canine samples. Springer Science + Business Media, Inc., 2007 haptoglobin nationallicence dog nationallicence validation nationallicence human immunoturbidimetric method nationallicence APP : acute-phase protein nationallicence EDTA : ethylenediaminetetraacetic acid nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence Hp : haptoglobin nationallicence Hb : haemoglobin nationallicence PBS : phosphate-buffered saline nationallicence Tecles F. Animal Medicine and Surgery Department, Veterinary School, University of Murcia, Murcia, Spain aut Subiela S. Animal Medicine and Surgery Department, Veterinary School, University of Murcia, Murcia, Spain aut Petrucci G. Veterinary School, Luteran University of Brazil, Porto Alegre, Brazil aut Panizo C. Animal Medicine and Surgery Department, Veterinary School, University of Murcia, Murcia, Spain aut Cerón J. Animal Medicine and Surgery Department, Veterinary School, University of Murcia, Murcia, Spain aut Veterinary Research Communications Kluwer Academic Publishers 31/1(2007-01-01), 23-36 0165-7380 31:1<23 2007 31 11259 https://doi.org/10.1007/s11259-006-3397-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-3397-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tecles F. Animal Medicine and Surgery Department, Veterinary School, University of Murcia, Murcia, Spain aut NATIONALLICENCE 700 1- Subiela S. Animal Medicine and Surgery Department, Veterinary School, University of Murcia, Murcia, Spain aut NATIONALLICENCE 700 1- Petrucci G. Veterinary School, Luteran University of Brazil, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Panizo C. Animal Medicine and Surgery Department, Veterinary School, University of Murcia, Murcia, Spain aut NATIONALLICENCE 700 1- Cerón J. Animal Medicine and Surgery Department, Veterinary School, University of Murcia, Murcia, Spain aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/1(2007-01-01), 23-36 0165-7380 31:1<23 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer