caa a22 4500 463237013 CHVBK 20180405153301.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s11259-007-0040-5 doi (NATIONALLICENCE)springer-10.1007/s11259-007-0040-5 Setting New Immunobiological Parameters in the Hamster Model of Visceral Leishmaniasis for In Vivo Testing of Antileishmanial Compounds [Elektronische Daten] [M. Dea-Ayuela, S. Rama-Íñiguez, J. Alunda, F. Bolás-Fernandez] To establish suitable immunobiological parameters for in vivo testing of new antileishmanial compounds in the golden hamster model of visceral leishmaniasis, two groups of 8 animals were infected each with 105 or 107 stationary promastigotes by the intracardiac route and the clinical and immunoparasitological features were monitored up to day 155 after infection. All animals became infected at both doses, although significant differences were observed between parasite burdens in liver and spleen. The mean number of parasites in animals infected with 107 promastigotes increased by 9.5 times in liver and by 43.1 times in spleen compared with those infected with 105 promastigotes. In animals given the higher dose, the outcome of the disease occurred between days 75 and 90 after infection, whereas no signs of disease were apparent in those given the lower infecting dose. Positive antibody (IgG) responses were detected earlier (week 5-7 after infection) in animals infected with the higher dose than in those infected with the lower dose (week 8-10 after infection), but these responses did not correlate with individual parasitological loads in liver and spleen. An inverse correlation was observed between infecting doses and in vitro spleen lymphocyte proliferation against mitogens (ConA). The proportion of CD4+ and CD19+ spleen cell increased in animals given the higher infection, whereas it decreased in those given the lower infection compared to naive controls. Springer Science + Business Media, Inc., 2007 Leishmania infantum nationallicence golden hamster nationallicence visceral infection nationallicence treatment nationallicence immunobiology nationallicence BSA : bovine serum albumin nationallicence CSE : crude saline extract nationallicence Con A : concanavalin A nationallicence EDTA : ethylenediaminetetraacetic acid nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence FACS : fluorescence-activated cell sorter nationallicence FCS : fetal calf serum nationallicence FELASA : Federation of European Laboratory Animal Associations nationallicence FITC : fluorescein isothiocyanate nationallicence IC : intracardiac nationallicence NNN : Novy, Nicolle and McNeal nationallicence OD : optical density nationallicence OPD : orthophenylenediamine nationallicence PBS : phosphate-buffered saline nationallicence Phyt : phytohaemagglutinin nationallicence R-PE : (R)-phycoerythrin nationallicence SI : stimulation index nationallicence Dea-Ayuela M. Departamento de Parasitología Facultad de Farmacia, Universidad Complutense, Madrid, Spain aut Rama-Íñiguez S. Departamento de Parasitología Facultad de Farmacia, Universidad Complutense, Madrid, Spain aut Alunda J. Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain aut Bolás-Fernandez F. Departamento de Parasitología Facultad de Farmacia, Universidad Complutense, Madrid, Spain aut Veterinary Research Communications Kluwer Academic Publishers 31/6(2007-08-01), 703-717 0165-7380 31:6<703 2007 31 11259 https://doi.org/10.1007/s11259-007-0040-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-0040-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dea-Ayuela M. Departamento de Parasitología Facultad de Farmacia, Universidad Complutense, Madrid, Spain aut NATIONALLICENCE 700 1- Rama-Íñiguez S. Departamento de Parasitología Facultad de Farmacia, Universidad Complutense, Madrid, Spain aut NATIONALLICENCE 700 1- Alunda J. Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain aut NATIONALLICENCE 700 1- Bolás-Fernandez F. Departamento de Parasitología Facultad de Farmacia, Universidad Complutense, Madrid, Spain aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/6(2007-08-01), 703-717 0165-7380 31:6<703 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer