caa a22 4500 463237021 CHVBK 20180405153301.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s11259-006-3565-0 doi (NATIONALLICENCE)springer-10.1007/s11259-006-3565-0 Comparative Pharmacokinetics of Gentamicin after Intravenous, Intramuscular, Subcutaneous and Oral Administration in Broiler Chickens [Elektronische Daten] [E. Abu-Basha, N. Idkaidek, A. Al-Shunnaq] The pharmacokinetics and bioavailability of gentamicin sulphate (5 mg/kg body weight) were studied in 50 female broiler chickens after single intravenous (i.v.), intramuscular (i.m.), subcutaneous (s.c.) and oral administration. Blood samples were collected at time 0 (pretreatment), and at 5, 15 and 30 min and 1, 2, 4, 6, 8, 12, 24 and 48 h after drug administration. Gentamicin concentrations were determined using a microbiological assay and Bacillus subtillis ATCC 6633 as a test organism. The limit of quantification was 0.2 μg/ml. The plasma concentration-time curves were analysed using non-compartmental methods based on statistical moment theory. Following i.v. administration, the elimination half-life (t 1/2β), the mean residence time (MRT), the volume of distribution at steady state (V ss), the volume of distribution (V d,area) and the total body clearance (ClB) were 2.93 ± 0.15 h, 2.08 ± 0.12 h, 0.77 ± 0.05 L/kg, 1.68 ± 0.39 L/kg and 5.06 ± 0.21 ml/min per kg, respectively. After i.m. and s.c. dosing, the mean peak plasma concentrations (C max) were 11.37 ± 0.73 and 16.65 ± 1.36 μg/ml, achieved at a post-injection times (t max) of 0.55 ± 0.05 and 0.75 ± 0.08 h, respectively. The t 1/2β was 2.87 ± 0.44 and 3.48 ± 0.37 h, respectively after i.m. and s.c. administration. The V d,area and ClB were 1.49 ± 0.21 L/kg and 6.18 ± 0.31 ml/min per kg, respectively, after i.m. administration and were 1.43 ± 0.19 L/kg and 4.7 ± 0.33 ml/min per kg, respectively, after s.c. administration. The absolute bioavailability (F) of gentamicin after i.m. administration was lower (79%) than that after s.c. administration (100%). Substantial differences in the resultant kinetics data were obtained between i.m. and s.c. administration. The in vitro protein binding of gentamicin in chicken plasma was 6.46%. Springer Science + Business Media, Inc., 2007 bioavailability nationallicence pharmacokinetics nationallicence chicken nationallicence gentamicin nationallicence Bacillus subtilis nationallicence AUC : area under plasma concentration-time curve nationallicence ClB : total body clearance nationallicence C max : maximum plasma concentration nationallicence t max : time to peak concentration nationallicence k el : elimination rate nationallicence V ss : volume of distribution at steady state nationallicence V d,area : volume of distribution nationallicence MRT : mean residence time nationallicence t 1/2β : elimination half-life nationallicence F : absolute bioavailability nationallicence MIC : minimum inhibitory concentration nationallicence i.v. : intravenous nationallicence i.m. : intramuscular nationallicence s.c. : subcutaneous nationallicence Abu-Basha E. Department of Veterinary Basic Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan aut Idkaidek N. Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan aut Al-Shunnaq A. Department of Veterinary Basic Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan aut Veterinary Research Communications Kluwer Academic Publishers 31/6(2007-08-01), 765-773 0165-7380 31:6<765 2007 31 11259 https://doi.org/10.1007/s11259-006-3565-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-3565-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abu-Basha E. Department of Veterinary Basic Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan aut NATIONALLICENCE 700 1- Idkaidek N. Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan aut NATIONALLICENCE 700 1- Al-Shunnaq A. Department of Veterinary Basic Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/6(2007-08-01), 765-773 0165-7380 31:6<765 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer