caa a22 4500 463237056 CHVBK 20180405153301.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s11259-007-3492-8 doi (NATIONALLICENCE)springer-10.1007/s11259-007-3492-8 Detection of Rabies Virus Genes by In-Situ Polymerase Chain Reaction [Elektronische Daten] [P. Praveena, R. Jayakumar, C. Balachandran, G. Thirumurugan, G. Raj, B. Manohar] Rabies is diagnosed by FAT in the impression smears of brain tissues. In this study, an attempt was made to diagnose rabies using in situ polymerase chain reaction (ISPCR). A digoxigenin-labelled double-stranded probe specific for a portion of the ‘N' gene of rabies virus was used. Positive signals were identified as blue dots in the intraneuronal and neuropil areas. Springer Science + Business Media, Inc., 2007 in situ PCR nationallicence rabies diagnosis nationallicence BSA : bovine serum albumin nationallicence DEPC : diethyl pyrocarbonate nationallicence dATP : deoxyadenosine triphosphate nationallicence dGTP : deoxyguanosine triphosphate nationallicence DIG : digoxigenin nationallicence cDNA : complementary DNA nationallicence DNase : deoxyribonuclease nationallicence dTTP : deoxythymiidne triphosphate nationallicence dUTP : deoxyuridine triphosphate nationallicence FAT : fluorescent antibody technique nationallicence ISPCR : in situ polymerase chain reaction nationallicence NBT-BCIP : 4-nitroblue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl phosphate nationallicence PCR : polymerase chain reaction nationallicence RNA : ribonucleic acid nationallicence RNase : ribonuclease nationallicence RT : reverse transcription nationallicence RT-PCR : reverse transcription-polymerase chain reaction nationallicence SSC : standard saline citrate nationallicence Tris : trimethoxyaminomethane nationallicence Praveena P. Indian Veterinary Research Institute, Izatnagar, Bareilly aut Jayakumar R. Department of Veterinary Epidemiology and Preventive Medicine, Chennai aut Balachandran C. Centralised Clinical Laboratory, Chennai aut Thirumurugan G. Vaccine Research Centre, Madhavaram Milk Colony, Chennai aut Raj G. Department of Animal Biotechnology, Chennai aut Manohar B. Department of Veterinary Pathology, Madras Veterinary College, Chennai aut Veterinary Research Communications Kluwer Academic Publishers 31/6(2007-08-01), 775-781 0165-7380 31:6<775 2007 31 11259 https://doi.org/10.1007/s11259-007-3492-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-3492-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Praveena P. Indian Veterinary Research Institute, Izatnagar, Bareilly aut NATIONALLICENCE 700 1- Jayakumar R. Department of Veterinary Epidemiology and Preventive Medicine, Chennai aut NATIONALLICENCE 700 1- Balachandran C. Centralised Clinical Laboratory, Chennai aut NATIONALLICENCE 700 1- Thirumurugan G. Vaccine Research Centre, Madhavaram Milk Colony, Chennai aut NATIONALLICENCE 700 1- Raj G. Department of Animal Biotechnology, Chennai aut NATIONALLICENCE 700 1- Manohar B. Department of Veterinary Pathology, Madras Veterinary College, Chennai aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/6(2007-08-01), 775-781 0165-7380 31:6<775 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer