caa a22 4500 463237072 CHVBK 20180405153301.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s11259-007-0057-9 doi (NATIONALLICENCE)springer-10.1007/s11259-007-0057-9 Culture and Stimulation of Tammar Wallaby Lymphocytes [Elektronische Daten] [L. Young, E. Deane] We describe the culture and stimulation of lymphocytes from the model marsupial, the tammar wallaby (Macropus eugenii). We also describe the capacity of tammar wallaby lymphocytes isolated from blood, spleen and lymph nodes to produce soluble immunomodulatory factors. Culture conditions were optimized for mitogen-driven stimulation using the plant lectin phytohaemagglutinin (PHA). Products secreted by stimulated cells were harvested and crudely fractionated before they were added back to freshly isolated lymphocytes. Using the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay, both stimulatory and inhibitory bioactive factors were detected in serum-free supernatants harvested from mitogen-treated peripheral blood mononuclear cells. This paper describes the capacity of leukocytes of the tammar wallaby to respond to mitogenic stimulation and to produce soluble, low-molecular-weight bioactive molecules that possess cytokine-like activity. Springer Science + Business Media, Inc., 2007 bioactivity nationallicence Macropus eugenii nationallicence marsupial nationallicence mitogen nationallicence PBMC nationallicence serum-free nationallicence stimulation nationallicence tammar wallaby nationallicence HBSS : Hanks balanced salt solution nationallicence MTT : 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide nationallicence PBMC : peripheral blood mononuclear cells nationallicence PHA : phytohaemagglutinin nationallicence PWM : pokeweed mitogen nationallicence SF : serum free nationallicence SI : stimulation index nationallicence [3H]thymidine : tritiated thymidine nationallicence Young L. School of Chemical and Biomedical Sciences, Central Queensland University, Rockhampton, Queensland aut Deane E. Division of Environmental and Life Sciences, Macquarie University, North Ryde, New South Wales, Australia aut Veterinary Research Communications Kluwer Academic Publishers 31/6(2007-08-01), 685-701 0165-7380 31:6<685 2007 31 11259 https://doi.org/10.1007/s11259-007-0057-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-0057-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Young L. School of Chemical and Biomedical Sciences, Central Queensland University, Rockhampton, Queensland aut NATIONALLICENCE 700 1- Deane E. Division of Environmental and Life Sciences, Macquarie University, North Ryde, New South Wales, Australia aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/6(2007-08-01), 685-701 0165-7380 31:6<685 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer