caa a22 4500 46323720X CHVBK 20180405153301.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s11259-007-0059-7 doi (NATIONALLICENCE)springer-10.1007/s11259-007-0059-7 Effect of Meiotic Stages During In Vitro Maturation on the Survival of Vitrified-Warmed Buffalo Oocytes [Elektronische Daten] [G. Sharma, K. Loganathasamy] The present study was conducted to investigate the effect of meiotic stages during in vitro maturation (IVM) on the survival of vitrified-warmed buffalo oocytes, vitrified at different stages of IVM. Cumulus oocyte complexes obtained from slaughterhouse ovaries were randomly divided into 6 groups: control (non-vitrified, matured for 24 h at 38 ± 1°C, 5% CO2 in humidified air), and those matured for 0 h (vitrified before IVM) or 6, 12, 18 and 24 h before vitrification. Cumulus oocyte complexes were vitrified in solution consisting of 40% w/v propylene glycol and 0.25 mol/L trehalose in phosphate-buffered saline supplemented with 4% w/v bovine serum albumin. Vitrified cumulus oocyte complexes were stored at −196∘C (liquid nitrogen) for at least 7 days and then thawed at 37°C; cryoprotectant was removed with 1 mol/L sucrose solution. Cumulus oocyte complexes in the 0, 6, 12, 18 and 24 h groups were then matured for an additional 24, 18, 12, 6 and 0 h, respectively, to complete 24 h of IVM. Among the five vitrification groups, 89-92% of cumulus oocyte complexes were recovered, after warming, of which 84-91% were morphologically normal. Overall survivability of vitrified cumulus oocyte complexes was lower (p < 0.05) than that of non-vitrified cumulus oocyte complexes (94.5%). Survival rates of cumulus oocyte complexes matured 24 h prior to vitrification (61.3%) were higher (p < 0.05) than those matured for 12 h (46.7%), 6 h (40.6%) and 0 h (37.6%). Nuclear status following 24 h IVM was assessed. A higher proportion of non-vitrified (control) oocytes (72.7%) reached metaphase II (M-II) stage in control than oocytes vitrified for 24 h (60.0%), 18 h (54.4), 12 h (42.3%), 6 h (33.3%) and 0 h (31.6%) (p < 0.05). The results suggest that length of time in maturation medium prior to vitrification influences post-thaw survivability of buffalo oocytes; longer intervals resulted in higher survival rates. Springer Science + Business Media, Inc., 2007 buffalo nationallicence oocytes nationallicence vitrification nationallicence propylene glycol nationallicence in vitro maturation nationallicence BSA : bovine serum albumin nationallicence COC : cumulus oocyte complexes nationallicence DMSO : dimethyl sulphoxide nationallicence EG : ethylene glycol nationallicence GDP : gross domestic product nationallicence IVM : in vitro maturation nationallicence MM : maturation medium nationallicence OCM : oocyte collection medium nationallicence PBS : phosphate-buffered saline nationallicence PROH : propylene glycol nationallicence VS : vitrification solution nationallicence Sharma G. Reproductive Physiology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, 243122, Izatnagar, U.P., India aut Loganathasamy K. Reproductive Physiology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, 243122, Izatnagar, U.P., India aut Veterinary Research Communications Springer Netherlands 31/7(2007-10-01), 881-893 0165-7380 31:7<881 2007 31 11259 https://doi.org/10.1007/s11259-007-0059-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-0059-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sharma G. Reproductive Physiology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, 243122, Izatnagar, U.P., India aut NATIONALLICENCE 700 1- Loganathasamy K. Reproductive Physiology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, 243122, Izatnagar, U.P., India aut NATIONALLICENCE 773 0- Veterinary Research Communications Springer Netherlands 31/7(2007-10-01), 881-893 0165-7380 31:7<881 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer