caa a22 4500 463237242 CHVBK 20180405153301.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s11259-007-0030-7 doi (NATIONALLICENCE)springer-10.1007/s11259-007-0030-7 Effects of Polyether Ionophores on the Protective Immune Responses of Broiler Chickens against Angara Disease and Newcastle Disease Viruses [Elektronische Daten] [K. Munir, M. Muneer, A. Tiwari, R. Chaudhry, S. Muruganandan] Immunization against Angara disease virus (ADV), a serotype 4 avian adenovirus, and Newcastle disease virus (NDV), an avian paramyxovirus serotype 1, is the mainstay of a broiler vaccination programme, while polyether ionophores usually form an essential component of a broiler medication programme in most parts of India and Pakistan. The role of polyether ionophores in the protective immune responses of broiler chickens vaccinated and challenged with ADV and NDV was investigated. A total of 1600 birds were divided into eight groups of 200 birds each. First four groups were vaccinated against NDV and ADV, while the remaining four served as unvaccinated controls. The first 3 groups of birds were administered salinomycin, monensin and cyclophosphamide (CYP), respectively. The last group served as an untreated control. The same treatment schedule was also followed for the next four unvaccinated groups. The post-vaccination and post-challenge serological responses to NDV and ADV, body and lymphoid organ weight gains, post-challenge survival rate and detection of NDV and ADV in the tissues of infected birds were evaluated. Birds administered salinomycin showed a significant stimulation of protective immune responses against both NDV and ADV as compared to the untreated and CYP-treated birds. Monensin also enhanced the protective immune responses against both viruses but the effect was not statistically significant. Thus, it is concluded that monensin and salinomycin augment the anti-NDV and anti-ADV immune responses in broiler chickens, which supports their use in poultry flocks. Springer Science + Business Media, Inc., 2007 angara disease virus nationallicence antibody response nationallicence chickens nationallicence immunomodulation nationallicence ionophores nationallicence mortality nationallicence newcastle disease virus nationallicence AAF : amnioallantoic fluids nationallicence ADV : Angara disease virus nationallicence AGP : agar gel precipitation nationallicence CPE : cytopathic effect(s) nationallicence CYP : cyclophosphamide nationallicence DNA : deoxyribonucleic acid nationallicence ED : eyedrop nationallicence EID50 : 50% embryo infective dose nationallicence HA : haemagglutination nationallicence HI : haemagglutination inhibition nationallicence IBD : infectious bursal disease nationallicence ICPI : intracerebral pathogenicity index nationallicence IM : intramuscular nationallicence IN : intranasal nationallicence IVPI : intravenous pathogenicity index nationallicence MDT : mean death time nationallicence NDV : Newcastle disease virus nationallicence PBS : phosphate-buffered saline nationallicence PCR : polymerase chain reaction nationallicence PHA : phytohaemagglutinin nationallicence p.i. : post infection nationallicence RNA : ribonucleic acid nationallicence RT : reverse transcription nationallicence SPF : specific pathogen-free nationallicence TCID50 : 50% tissue culture infective dose nationallicence VI : virus isolation nationallicence Munir K. University of Veterinary and Animal Sciences, Lahore, Pakistan aut Muneer M. University of Veterinary and Animal Sciences, Lahore, Pakistan aut Tiwari A. Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada aut Chaudhry R. Lahore Veterinary Diagnostic Laboratories, Shadman, Lahore, Pakistan aut Muruganandan S. Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada aut Veterinary Research Communications Springer Netherlands 31/7(2007-10-01), 909-929 0165-7380 31:7<909 2007 31 11259 https://doi.org/10.1007/s11259-007-0030-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-0030-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Munir K. University of Veterinary and Animal Sciences, Lahore, Pakistan aut NATIONALLICENCE 700 1- Muneer M. University of Veterinary and Animal Sciences, Lahore, Pakistan aut NATIONALLICENCE 700 1- Tiwari A. Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada aut NATIONALLICENCE 700 1- Chaudhry R. Lahore Veterinary Diagnostic Laboratories, Shadman, Lahore, Pakistan aut NATIONALLICENCE 700 1- Muruganandan S. Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada aut NATIONALLICENCE 773 0- Veterinary Research Communications Springer Netherlands 31/7(2007-10-01), 909-929 0165-7380 31:7<909 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer