caa a22 4500 463237269 CHVBK 20180405153302.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s11259-007-0004-9 doi (NATIONALLICENCE)springer-10.1007/s11259-007-0004-9 Upregulation of TNF-α Production by IFN-γ and LPS in Cultured Canine Keratinocytes: Application to Monosaccharides Effects [Elektronische Daten] [C. Ibisch, P. Bourdeau, C. Cadiot, J. Viac, H. Gatto] Activated keratinocytes play a key role in the cutaneous immune system by their interactions with other cell types through the production of cytokines with both autocrine and paracrine activity. But there is little knowledge about epidermal cytokines in the dog. In this study, cultured canine keratinocytes were stimulated by human recombinant interferon γ (IFN-γ) and lipopolysaccharide (LPS) and cell supernatants were tested for tumour necrosis factor α (TNF-α) concentration using a cell viability assay on a murine cell line. We show that IFN-γ in combination with LPS significantly increases TNF-α secretion by canine keratinocytes. The best stimulations were obtained using confluent cultures and the association of IFN-γ (400 ng/ml) and LPS (40 μg/ml). The experimental protocol we describe represents a new method for studying keratinocyte activation and its modulation in the dog. We provide an example of application of our method: the study of the effects of different monosaccharides on canine keratinocyte activation. Springer Science + Business Media, Inc., 2007 arabinose nationallicence canine keratinocyte activation nationallicence cell culture nationallicence fucose nationallicence IFN-γ nationallicence rhamnose nationallicence TNF-α nationallicence IFN : interferon nationallicence LPS : lipopolysaccharide nationallicence MTT : 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide nationallicence OD : optical density nationallicence SDS : sodium dodecyl sulphate nationallicence TNF : tumour necrosis factor nationallicence Ibisch C. Unit of Dermatology, Clinical Parasitology and Mycology, National Veterinary School, Atlanpole - La Chantrerie, BP 40 706, 44 307, Nantes Cedex 03, France aut Bourdeau P. Unit of Dermatology, Clinical Parasitology and Mycology, National Veterinary School, Atlanpole - La Chantrerie, BP 40 706, 44 307, Nantes Cedex 03, France aut Cadiot C. Unit of Dermatology, Clinical Parasitology and Mycology, National Veterinary School, Atlanpole - La Chantrerie, BP 40 706, 44 307, Nantes Cedex 03, France aut Viac J. Unité INSERM 346, Hôpital E. Herriot, Lyon Cedex, France aut Gatto H. Virbac S.A. Laboratories, Carros, France aut Veterinary Research Communications Springer Netherlands 31/7(2007-10-01), 835-846 0165-7380 31:7<835 2007 31 11259 https://doi.org/10.1007/s11259-007-0004-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-0004-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ibisch C. Unit of Dermatology, Clinical Parasitology and Mycology, National Veterinary School, Atlanpole - La Chantrerie, BP 40 706, 44 307, Nantes Cedex 03, France aut NATIONALLICENCE 700 1- Bourdeau P. Unit of Dermatology, Clinical Parasitology and Mycology, National Veterinary School, Atlanpole - La Chantrerie, BP 40 706, 44 307, Nantes Cedex 03, France aut NATIONALLICENCE 700 1- Cadiot C. Unit of Dermatology, Clinical Parasitology and Mycology, National Veterinary School, Atlanpole - La Chantrerie, BP 40 706, 44 307, Nantes Cedex 03, France aut NATIONALLICENCE 700 1- Viac J. Unité INSERM 346, Hôpital E. Herriot, Lyon Cedex, France aut NATIONALLICENCE 700 1- Gatto H. Virbac S.A. Laboratories, Carros, France aut NATIONALLICENCE 773 0- Veterinary Research Communications Springer Netherlands 31/7(2007-10-01), 835-846 0165-7380 31:7<835 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer