caa a22 4500 463237307 CHVBK 20180405153302.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s11259-006-3434-x doi (NATIONALLICENCE)springer-10.1007/s11259-006-3434-x Potential Aflatoxin and Ochratoxin A Production by Aspergillus Species in Poultry Feed Processing [Elektronische Daten] [M. Fraga, F. Curvello, M. Gatti, L. Cavaglieri, A. Dalcero, C. da Rocha Rosa] Poultry feeds are prone to fungal growth and mycotoxin production during processing. The identification of biota with the ability to produce mycotoxins is essential. The aims of this study were (1) to monitor the mycobiota counts at different stages of poultry feed processing; (2) to determine the occurrence of Aspergillus species; (3) to evaluate the natural incidence of aflatoxins and ochratoxin A. The ability of Aspergillus spp. and its teleomorphs isolated here to produce these toxins was also investigated. Samples (144) were collected at random from a factory in Brazil. The occurrence of Aspergillus and Eurotium species was demonstrated on DRBC and DG18 media and the production of aflatoxins and ochratoxin A and their natural incidence were determined by TLC and HPLC methods. A. flavus and E. chevalieri were the most prevalent species isolated. Fungal contamination was not found after the pelleting process, though Aspergillus and Eurotium species were recovered from trough samples. High levels of aflatoxin and ochratoxin A producers were found at all stages of poultry feed processing. Also, high natural contamination with aflatoxins and ochratoxin A was found in the samples. Contact of feed with remainder poultry feed could lead to fungal contamination, so the risk of aflatoxin and/or ochratoxin A contamination of feed must be taken into account. Springer Science + Business Media, Inc., 2007 aflatoxins nationallicence Aspergillus nationallicence Eurotium nationallicence ochratoxin A nationallicence poultry feed nationallicence AF : aflatoxin nationallicence DG18 : dichloran-18% glycerol agar nationallicence DRBC : dichloran-rose bengal-chloramphenicol agar nationallicence HPLC : high-pressure liquid chromatography nationallicence OTA : ochratoxin A nationallicence TLC : thin-layer chromatography nationallicence Fraga M. Departamento de Microbiologia e Imunología Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro aut Curvello F. Departamento de Produção Animal, Universidade Federal Rural do Rio de Janeiro, Instituto de Zootecnia, Rio de Janeiro aut Gatti M. Departamento de Biologia, Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil aut Cavaglieri L. Departamento de Microbiologia e Inmunología, Universidad Nacional de Rio Cuarto, Rio Cuarto, Argentina aut Dalcero A. Departamento de Microbiologia e Inmunología, Universidad Nacional de Rio Cuarto, Rio Cuarto, Argentina aut da Rocha Rosa C. Departamento de Microbiologia e Imunología Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro aut Veterinary Research Communications Kluwer Academic Publishers 31/3(2007-04-01), 343-353 0165-7380 31:3<343 2007 31 11259 https://doi.org/10.1007/s11259-006-3434-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-3434-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fraga M. Departamento de Microbiologia e Imunología Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro aut NATIONALLICENCE 700 1- Curvello F. Departamento de Produção Animal, Universidade Federal Rural do Rio de Janeiro, Instituto de Zootecnia, Rio de Janeiro aut NATIONALLICENCE 700 1- Gatti M. Departamento de Biologia, Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil aut NATIONALLICENCE 700 1- Cavaglieri L. Departamento de Microbiologia e Inmunología, Universidad Nacional de Rio Cuarto, Rio Cuarto, Argentina aut NATIONALLICENCE 700 1- Dalcero A. Departamento de Microbiologia e Inmunología, Universidad Nacional de Rio Cuarto, Rio Cuarto, Argentina aut NATIONALLICENCE 700 1- da Rocha Rosa C. Departamento de Microbiologia e Imunología Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/3(2007-04-01), 343-353 0165-7380 31:3<343 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer