caa a22 4500 463237331 CHVBK 20180405153302.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s11259-006-3442-x doi (NATIONALLICENCE)springer-10.1007/s11259-006-3442-x Development of an Indirect ELISA for the Detection of Antibodies against Peste-des-petits-ruminants Virus in Small Ruminants [Elektronische Daten] [V. Balamurugan, R. Singh, P. Saravanan, A. Sen, J. Sarkar, B. Sahay, T. Rasool, R. Singh] Peste des petits ruminants (PPR) is an acute, febrile, highly contagious and economically important viral disease of small ruminants. A polyclonal antibody based indirect ELISA was developed for detection of antibodies to PPR virus in the serum samples of goats and sheep using purified PPR viral antigen propogated in Vero cell culture. A threshold (cut-off) value was set as twice the mean of the negative population based on the distribution of known negative serum samples in respect of PPR virus antibodies in the test. A total of 1544 serum samples from goats and sheep were screened by indirect ELISA and competitive ELISA. The indirect ELISA compared very well with competitive ELISA, with a high degree of specificity (95.09%) and sensitivity (90.81%). When compared with virus neutralization test, the present assay had 100% specificity and 80% sensitivity. With serum samples, the assay could clearly differentiate animals from the infected population from uninfected ones. These results suggest that the indirect ELISA may be a good alternative tool to competitive ELISA for seroepidemiological surveys. Springer Science + Business Media, Inc., 2007 indirect-ELISA nationallicence peste des petits ruminants nationallicence antibody detection nationallicence small ruminants nationallicence c-ELISA : competitive enzyme-linked immunosorbent assay nationallicence CPE : cytopathic effect nationallicence HRPO : horseradish peroxidase nationallicence OD : optical density nationallicence OPD : orthophenylenediamine nationallicence PEG : polyethylene glycol nationallicence PPR : peste des petits ruminants nationallicence VNT : virus neutralization test nationallicence Balamurugan V. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut Singh R. Division of Biological Products, Indian Veterinary Research Institute, Izatnagar, Bareilly, India aut Saravanan P. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut Sen A. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut Sarkar J. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut Sahay B. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut Rasool T. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut Singh R. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut Veterinary Research Communications Kluwer Academic Publishers 31/3(2007-04-01), 355-364 0165-7380 31:3<355 2007 31 11259 https://doi.org/10.1007/s11259-006-3442-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-3442-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Balamurugan V. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut NATIONALLICENCE 700 1- Singh R. Division of Biological Products, Indian Veterinary Research Institute, Izatnagar, Bareilly, India aut NATIONALLICENCE 700 1- Saravanan P. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut NATIONALLICENCE 700 1- Sen A. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut NATIONALLICENCE 700 1- Sarkar J. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut NATIONALLICENCE 700 1- Sahay B. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut NATIONALLICENCE 700 1- Rasool T. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut NATIONALLICENCE 700 1- Singh R. Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/3(2007-04-01), 355-364 0165-7380 31:3<355 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer