caa a22 4500 463237374 CHVBK 20180405153302.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s11259-006-3441-y doi (NATIONALLICENCE)springer-10.1007/s11259-006-3441-y Distribution and Expression of Recombinant Plasmid Encoding Chicken Interleukin-2 [Elektronische Daten] [Z. You, L. Yu, C. Zhang, L. Li, M. Lu, Z. Mao, Y. Liu, W. Chu] A plasmid DNA that encodes chicken interleukin-2 (pCI-ChIL-2-EGFP) was investigated for its distribution and expression after intramuscular (i.m.) injection in chickens. After the i.m. injection, serum distribution was detectable from 2 h post inoculation (p.i.), peaked at 8 h p.i., and disappeared at 7 days p.i. The plasmid DNA was also observed in several organs including heart, liver, lung, spleen, bursa and inoculated muscle at different time points, but at 19 days p.i. the plasmid DNA was not found in any organ except inoculated muscle. Fluorescence of enhanced green fluorescent protein (EGFP) was found in cytoplasm and nucleus of cultured Vero cells, chicken embryo fibroblasts and peripheral blood lymphocytes, which were transfected in vitro with the plasmid DNA or in vivo with Lipofectamine. The expression profile of the fusion gene (ChIL-2-EGFP) in vivo was measured by RT-PCR, ELISA and fluorescence microscopy. The EGFP expression was detected from 8 h p.i. to 14 days p.i. and peaked at 5 days p.i., when the number of EGFP-expression myocytes was about 5% in the injected site. These results demonstrate that intramuscular administration of plasmid DNA leads to widespread distribution and long-term expression in vivo. Springer Science + Business Media, Inc., 2007 chicken interleukin 2 (ChIL-2) nationallicence recombinant plasmid nationallicence distribution nationallicence enhanced green fluorescence protein (EGFP) nationallicence expression nationallicence CEF : chicken embryo fibroblasts nationallicence ChIL-2 : chicken interleukin-2 nationallicence DCs : dendritic cells nationallicence EGFP : enhanced green fluorescent protein nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence HRP : hevseradish peroxidose nationallicence i.m. : intramuscular nationallicence OD : optical density nationallicence ORF : open reading frame nationallicence PBLC : periphery blood lymphocytes nationallicence PBS : phosphate-buffered saline nationallicence PCR : polymerase chain reaction nationallicence p.i. : post inoculation nationallicence p.t. : post transfection nationallicence RT : reverse transcription nationallicence You Z. Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, China aut Yu L. Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, China aut Zhang C. Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, China aut Li L. Department of Biomedical Engineering, Zhejiang University, Hangzhou, China aut Lu M. Zhejiang Academy of Medical Science, Hangzhou, China aut Mao Z. Department of Biomedical Engineering, Zhejiang University, Hangzhou, China aut Liu Y. Department of Biomedical Engineering, Zhejiang University, Hangzhou, China aut Chu W. Department of Biomedical Engineering, Zhejiang University, Hangzhou, China aut Veterinary Research Communications Kluwer Academic Publishers 31/3(2007-04-01), 273-285 0165-7380 31:3<273 2007 31 11259 https://doi.org/10.1007/s11259-006-3441-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-3441-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- You Z. Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, China aut NATIONALLICENCE 700 1- Yu L. Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, China aut NATIONALLICENCE 700 1- Zhang C. Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, China aut NATIONALLICENCE 700 1- Li L. Department of Biomedical Engineering, Zhejiang University, Hangzhou, China aut NATIONALLICENCE 700 1- Lu M. Zhejiang Academy of Medical Science, Hangzhou, China aut NATIONALLICENCE 700 1- Mao Z. Department of Biomedical Engineering, Zhejiang University, Hangzhou, China aut NATIONALLICENCE 700 1- Liu Y. Department of Biomedical Engineering, Zhejiang University, Hangzhou, China aut NATIONALLICENCE 700 1- Chu W. Department of Biomedical Engineering, Zhejiang University, Hangzhou, China aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/3(2007-04-01), 273-285 0165-7380 31:3<273 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer