caa a22 4500 463238346 CHVBK 20180405153305.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11259-007-3539-x doi (NATIONALLICENCE)springer-10.1007/s11259-007-3539-x Assessment of Lactate Dehydrogenase, Alkaline Phosphatase and Aspartate Aminotransferase Activities in Cow's Milk as an Indicator of Subclinical Mastitis [Elektronische Daten] [H. Babaei, L. Mansouri-Najand, M. Molaei, A. Kheradmand, M. Sharifan] This study examined the activities of lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in the milk of lactating Holstein cows in association with subclinical mastitis (SCM). A total of 94 milk samples were collected from 58 lactating dairy cows representing stages of lactation from the second to the tenth week after calving. Those which were classified as positive by California mastitis test (CMT) were deemed to have subclinical mastitis. All the milk samples were skimmed by centrifugation at 10 000g at 0°C and were used for enzyme activities estimations. The mean activities of LDH and ALP were higher in the milk from udders with SCM than in the milk from healthy udders p < 0.05). There were no significant differences in AST values. The maximum agreement rates between the CMT results and LDH and ALP values were seen at thresholds of >180 IU/L and >40 IU/L respectively (κ values 0.65 and 0.79, respectively). However, the sensitivity of the tests for identifying SCM at these thresholds was higher for ALP (96.4%) than for LDH (68.5%). In this study, LDH and ALP tests were standardized for cow's milk and results showed that only the ALP test was reliable in the early diagnosis of subclinical mastitis. Springer Science + Business Media, Inc., 2007 alkaline phosphatase nationallicence aspartate aminotransferase nationallicence dairy cow nationallicence lactate dehydrogenase nationallicence nonlysosomal enzymes nationallicence subclinical mastitis nationallicence LDH : lactate dehydrogenase nationallicence ALP : alkaline phosphatase nationallicence AST : aspartate aminotransferase nationallicence SCM : subclinical mastitis nationallicence CMT : California mastitis test nationallicence SCC : somatic cell count nationallicence PPV : positive predictive value nationallicence NPV : negative predictive value nationallicence Babaei H. Theriogenology Section, Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran aut Mansouri-Najand L. Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran aut Molaei M. Theriogenology Section, Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran aut Kheradmand A. School of Veterinary Medicine, Lorestan University, Khorram Abad, Iran aut Sharifan M. aut Veterinary Research Communications Kluwer Academic Publishers 31/4(2007-05-01), 419-425 0165-7380 31:4<419 2007 31 11259 https://doi.org/10.1007/s11259-007-3539-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-3539-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Babaei H. Theriogenology Section, Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran aut NATIONALLICENCE 700 1- Mansouri-Najand L. Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran aut NATIONALLICENCE 700 1- Molaei M. Theriogenology Section, Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran aut NATIONALLICENCE 700 1- Kheradmand A. School of Veterinary Medicine, Lorestan University, Khorram Abad, Iran aut NATIONALLICENCE 700 1- Sharifan M. aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/4(2007-05-01), 419-425 0165-7380 31:4<419 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer