caa a22 4500 463238362 CHVBK 20180405153305.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11259-006-3390-5 doi (NATIONALLICENCE)springer-10.1007/s11259-006-3390-5 Polymerase Chain Reaction-Restriction Fragment Length Polymorphism of Mitochondrial 12S rRNA Gene: A Simple Method for Identification of Poultry Meat Species [Elektronische Daten] [P. Girish, A. Anjaneyulu, K. Viswas, F. Santhosh, K. Bhilegaonkar, R. Agarwal, N. Kondaiah, K. Nagappa] Chicken (Gallus gallus), duck (Anas platyrhynchos), turkey (Meleagris gallopavo), guinea fowl (Numida meleagris) and quail (Coturnix japonica) are the common poultry species consumed as meat throughout the world. In this work, a molecular technique has been developed for identification and differentiation of meat originating from these species. This tool helps in detection of misrepresentation of different poultry meats. The technique involves the extraction of DNA from the given sample, polymerase chain reaction (PCR) amplification of mitochondrial 12S rRNA gene using universal primers, restriction analysis with selected restriction enzymes, followed by identification of meat species based on restriction fragment length polymorphism (RFLP) pattern. In this study, we used HinfI, Mph1103I, MvaI, and Eco47I to identify and differentiate to poultry species referred to above. This species identification technique has also been applied successfully to processed meat products including those cooked at 120∘C for 30 min. Simplicity of interpretation of results combined with versatility makes this a convenient and appropriate technique in the hands of meat analysts for identifying poultry meat species. Springer Science + Business Media, Inc., 2007 12S rRNA gene nationallicence meat species nationallicence PCR nationallicence poultry nationallicence RFLP nationallicence bp : base pairs nationallicence dNTP : deoxynucleotide triphosphate nationallicence DNAase : deoxyribonuclease nationallicence DTT : Dithiothreitol nationallicence EDTA : ethylenediaminetetraacetate nationallicence mt : mitochondrial nationallicence PCR : polymerase chain reaction nationallicence reak RAPD, random amplified polymorphic DNA nationallicence RE : restriction enzyme nationallicence RFLP : restriction fragment length polymorphism nationallicence RNase : riboendonuclease nationallicence SDS : sodium dodecyl sulphate nationallicence TE buffer : Tris-EDTA buffer nationallicence Girish P. Division of Livestock Products Technology, Indian Veterinary Research Institute, Izatnagar aut Anjaneyulu A. Division of Livestock Products Technology, Indian Veterinary Research Institute, Izatnagar aut Viswas K. National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar aut Santhosh F. Central Avian Research Institute, Indian Veterinary Research Institute, Izatnagar aut Bhilegaonkar K. Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar aut Agarwal R. Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar aut Kondaiah N. Division of Livestock Products Technology, Indian Veterinary Research Institute, Izatnagar aut Nagappa K. Department of Verterinary Public Health, C.V.A.Sc, G.B.P.U.A. & T, Pantnagar, India aut Veterinary Research Communications Kluwer Academic Publishers 31/4(2007-05-01), 447-455 0165-7380 31:4<447 2007 31 11259 https://doi.org/10.1007/s11259-006-3390-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-3390-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Girish P. Division of Livestock Products Technology, Indian Veterinary Research Institute, Izatnagar aut NATIONALLICENCE 700 1- Anjaneyulu A. Division of Livestock Products Technology, Indian Veterinary Research Institute, Izatnagar aut NATIONALLICENCE 700 1- Viswas K. National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar aut NATIONALLICENCE 700 1- Santhosh F. Central Avian Research Institute, Indian Veterinary Research Institute, Izatnagar aut NATIONALLICENCE 700 1- Bhilegaonkar K. Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar aut NATIONALLICENCE 700 1- Agarwal R. Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar aut NATIONALLICENCE 700 1- Kondaiah N. Division of Livestock Products Technology, Indian Veterinary Research Institute, Izatnagar aut NATIONALLICENCE 700 1- Nagappa K. Department of Verterinary Public Health, C.V.A.Sc, G.B.P.U.A. & T, Pantnagar, India aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/4(2007-05-01), 447-455 0165-7380 31:4<447 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer