caa a22 4500 463238389 CHVBK 20180405153305.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11259-007-3469-7 doi (NATIONALLICENCE)springer-10.1007/s11259-007-3469-7 Immunogenicity of Empty Capsids of Porcine Circovius Type 2 Produced in Insect Cells [Elektronische Daten] [H. Fan, C. Ju, T. Tong, H. Huang, J. Lv, H. Chen] Porcine circovirus type 2 (PCV2), a single-stranded DNA virus, is associated with postweaning multisystemic wasting syndrome (PMWS). ORF2 protein (capsid) of PCV2 was recently demonstrated to be a major immunogenable to induce protection in pigs with a prime-boost protocol. In this study, the ORF2 gene of PCV2 was expressed in insect cells. The product self-assembled into particles that were structurally and antigenically indistinguishable from regular PCV2 capsids. To evaluated the immunogenicity of these virus-like particles, PCV2-free piglets were vaccinated with the crude lysate from recombinant baculovirus (Ac.ORF2)-infected insect cells, at doses of 0.1 ml (106 cells), 0.5 ml (5 × 106 cells) or 1.0 ml (107 cells). The immune response was monitored by an indirect enzyme-linked immunosorbent assay (ELISA) for PCV2 antibody and lymphocyte proliferation assay. The ELISA results indicated that primary immune response was elicited with 0.5 ml or 1.0 ml of crude lysate from Ac.ORF2. After boost immunization, relatively higher levels of PCV2 antibody were elicited in 0.5-ml or 1.0-ml vaccinated groups, compared to the 0.1-ml group. In addition, higher PCV2 specific lymphocyte proliferation response was developed in piglets vaccinated with 0.5 ml or 1.0 ml of crude lysate, especially in those vaccinated with with 1.0 ml of crude lysate. Thus, the expressed ORF2 protein has significant potential as a subunit vaccine against PCV2 infection. Springer Science + Business Media, Inc., 2007 ORF2 nationallicence porcine circovirus type 2 (PCV2) nationallicence postweaning multisystemic wasting syndrome nationallicence PMWS subunit vaccine nationallicence AcNPV : Autographa california nuclear ployhedrosis virus nationallicence Ac.ORF2 : recombinant AcNPV expressing ORF2 protein nationallicence CTL : cytotoxic lymphocyte nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence HRP : horseradish peroxidase nationallicence MHC : major histocompatibility complex nationallicence PBMC : peripheral blood mononuclear cell nationallicence PCR : polymerase chain reaction nationallicence PCV : porcine circovirus nationallicence pfu : plaque-forming units nationallicence p.i. : post infection nationallicence PMWS : post-weaning multisystemic wasting syndrome nationallicence PTA : phosphotungstic acid nationallicence Sf9 : Spodoptera frugiperda nationallicence SI : stimulation index nationallicence VLP : virus-like particle nationallicence wt. AcNPV : wild-type Autographa california nuclear polyhedrosis virus nationallicence Fan H. Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China aut Ju C. Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China aut Tong T. Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China aut Huang H. Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China aut Lv J. Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China aut Chen H. Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China aut Veterinary Research Communications Kluwer Academic Publishers 31/4(2007-05-01), 487-496 0165-7380 31:4<487 2007 31 11259 https://doi.org/10.1007/s11259-007-3469-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-3469-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fan H. Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China aut NATIONALLICENCE 700 1- Ju C. Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China aut NATIONALLICENCE 700 1- Tong T. Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China aut NATIONALLICENCE 700 1- Huang H. Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China aut NATIONALLICENCE 700 1- Lv J. Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China aut NATIONALLICENCE 700 1- Chen H. Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/4(2007-05-01), 487-496 0165-7380 31:4<487 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer