caa a22 4500 463238419 CHVBK 20180405153305.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11259-006-3448-4 doi (NATIONALLICENCE)springer-10.1007/s11259-006-3448-4 Experimental Infection of Pigs with ‘ Candidatus Helicobacter suis' [Elektronische Daten] [A. Hellemans, K. Chiers, A. Decostere, M. Bock, F. Haesebrouck, R. Ducatelle] ‘Candidatus Helicobacter suis' is a spiral-shaped bacterium that colonizes the stomach of more than 60% of slaughter pigs. The role of ‘Candidatus Helicobacter suis' in gastric disease of pigs is still unclear. Experimental studies in pigs are lacking because this bacterium is unculturable until now. An inoculation protocol using ‘Candidatus Helicobacter suis' infected mouse stomach homogenate was used to reproduce the infection in pigs. Control animals were inoculated using negative mouse stomach homogenate. Pigs were inoculated three times with one-week intervals and euthanized 6 weeks post inoculation. Tissue samples were taken from different mucosal stomach regions to detect ‘Candidatus Helicobacter suis' by PCR and urease test. Mucosal inflammation was evaluated on formalin-fixed tissue samples. Lesions in the pars oesophagea were scored macroscopically. Infection was succesful in all challenged animals, with the antrum and the fundus being predominantly positive. Infection was associated with infiltration of lymphocytes and plasma cells in the antral mucosa, evolving to follicular gastritis. No apparent inflammation of the fundic stomach region was detected in the infected animals. A clear link between ‘Candidatus Helicobacter suis' and pars oesophageal lesions could not be found. Springer Science + Business Media, Inc., 2007 ‘ Candidatus Helicobacter suis' nationallicence gastritis nationallicence gastric ulcer nationallicence in vivo model nationallicence pigs nationallicence Helicobacter heilmannii nationallicence BHI : brain-heart infusion nationallicence LYM : lyophilization medium nationallicence OD : optical density nationallicence PI : Post inoculation nationallicence SPF : specific pathogen-free nationallicence Hellemans A. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium aut Chiers K. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium aut Decostere A. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium aut Bock M. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium aut Haesebrouck F. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium aut Ducatelle R. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium aut Veterinary Research Communications Kluwer Academic Publishers 31/4(2007-05-01), 385-395 0165-7380 31:4<385 2007 31 11259 https://doi.org/10.1007/s11259-006-3448-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-3448-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hellemans A. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium aut NATIONALLICENCE 700 1- Chiers K. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium aut NATIONALLICENCE 700 1- Decostere A. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium aut NATIONALLICENCE 700 1- Bock M. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium aut NATIONALLICENCE 700 1- Haesebrouck F. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium aut NATIONALLICENCE 700 1- Ducatelle R. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/4(2007-05-01), 385-395 0165-7380 31:4<385 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer