caa a22 4500 463238443 CHVBK 20180405153305.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s11259-006-0164-z doi (NATIONALLICENCE)springer-10.1007/s11259-006-0164-z Onaga T. Laboratory of Veterinary Physiology, Department of Biosciences, School of Veterinary Medicine, Rakuno Gakuen University, Japan aut Effect of intravenous infusion of proglumide on ruminal motility in conscious sheep ( Ovis aries ) [Elektronische Daten] [T. Onaga] The effects of intravenous infusion of proglumide on regular ruminal contractions were examined in conscious sheep using doses that inhibit pancreatic exocrine secretion. After a control period of 20 min, proglumide was infused intravenously for 40 min at a dose of 15, 30 or 60 μmol/kg per min and venous blood was collected. The intravenous infusion of proglumide significantly increased the frequency of ruminal contractions at 15 μmol/kg per min without altering the amplitude, while it significantly decreased the frequency and amplitude of ruminal contractions at 30 and 60 μmol/kg per min in a dose-dependent manner. Proglumide did not increase contractile activity of the omasum, abomasum and duodenum or the plasma concentration of immunoreactive cholecystokinin (CCK). Application of proglumide at 1-30 mmol/L inhibited bethanechol-induced contraction in both longitudinal and circular muscle strips of the dorsal sac of the rumen. These results suggest that proglumide at a low dose acts indirectly on the rumen as a CCK receptor antagonist to increase the frequency of contractions, whereas at higher doses it inhibits cholinergic-induced contraction of the ruminal muscles or acts as an agonist to inhibit contractions in sheep. Hence, proglumide at high doses seems unsuitable for research or therapeutic use as a CCK receptor blockade in sheep. Springer Science + Business Media, Inc., 2007 CCK receptor antagonist nationallicence reticulo-ruminal motility nationallicence ovine rumen nationallicence ruminant nationallicence BCh : bethanechol nationallicence CCK : cholecystokinin nationallicence i.c.v. : intracerebroventricular nationallicence i.v. : intravenous nationallicence Veterinary Research Communications Springer Netherlands 31/8(2007-11-01), 1021-1036 0165-7380 31:8<1021 2007 31 11259 https://doi.org/10.1007/s11259-006-0164-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-0164-z text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Onaga T. Laboratory of Veterinary Physiology, Department of Biosciences, School of Veterinary Medicine, Rakuno Gakuen University, Japan aut NATIONALLICENCE 773 0- Veterinary Research Communications Springer Netherlands 31/8(2007-11-01), 1021-1036 0165-7380 31:8<1021 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer