caa a22 4500 463238478 CHVBK 20180405153305.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s11259-007-0033-4 doi (NATIONALLICENCE)springer-10.1007/s11259-007-0033-4 Slide Enzyme-Linked Immunosorbent Assay for the Diagnosis of Babesia bigemina Infection in Bovines [Elektronische Daten] [R. Ravindran, A. Mishra, J. Rao] A slide enzyme-linked immunosorbent assay (SELISA) for the diagnosis of Babesia bigemina infection in cattle was standardized. Acetone-fixed whole Babesia bigemina-infected erythrocytes on micro-slides were immunoreacted with bovine serum samples followed by antibovine horseradish peroxidase conjugate and developed using diaminobenzidine tetrahydrochloride as a substrate. The positive immunoreactivity (staining pattern) was visualized in the form of dark brown piroplasms. Using the laboratory-standardized SELISA with a sensitivity of 94.4%, the seroprevalence of babesiosis was studied in cattle from two endemic areas of the disease. In comparison to IFAT, SELISA detected higher number of serum samples positive for bovine babesiosis. Springer Science + Business Media, Inc., 2007 Babesia bigemina nationallicence slide enzyme-linked immunosorbent assay nationallicence DAB : diaminobenzidine tetrahydrochloride nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence IFAT : indirect fluorescent antibody test nationallicence PBS : phosphate-buffered saline nationallicence RBC : red blood corpuscle nationallicence SELISA : slide enzyme-linked immunosorbent assay nationallicence Ravindran R. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India aut Mishra A. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India aut Rao J. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India aut Veterinary Research Communications Springer Netherlands 31/8(2007-11-01), 999-1004 0165-7380 31:8<999 2007 31 11259 https://doi.org/10.1007/s11259-007-0033-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-0033-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ravindran R. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India aut NATIONALLICENCE 700 1- Mishra A. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India aut NATIONALLICENCE 700 1- Rao J. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India aut NATIONALLICENCE 773 0- Veterinary Research Communications Springer Netherlands 31/8(2007-11-01), 999-1004 0165-7380 31:8<999 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer