caa a22 4500 463238494 CHVBK 20180405153305.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s11259-006-0201-y doi (NATIONALLICENCE)springer-10.1007/s11259-006-0201-y Complement Receptor Type 3 (CR3)- and Fc Receptor (FcR)-Mediated Matrix Metalloproteinase 9 (MMP-9) Secretion and Their Intracellular Signalling of Bovine Neutrophils [Elektronische Daten] [H. Higuchi, M. Ishizaka, H. Nagahata] Complement receptor type 3 (CR3)- and Fc receptor (FcR)-mediated metalloproteinase-9 (MMP-9) secretion and their intracellular signalling of bovine neutrophils were evaluated. Relative density of MMP-9 secreted by neutrophils stimulated with opsonized zymosan (OPZ, stimulant for CR3) was significantly (p < 0.05) increased when the OPZ concentration was increased from 0 to 0.4 mg/ml. Similar results were obtained for neutrophils stimulated with heat-aggregated IgG (Agg-IgG, stimulant for Fc receptor) at concentrations from 0 to 0.40 mg/ml. Preincubation of neutrophils with 1-30 nmol/L wortmannin (phosphoinositide 3-kinase inhibitor) resulted in inhibition of MMP-9 secretion induced by stimulation with OPZ and Agg-IgG in a concentration-dependent manner, 30 nmol/L wortmannin causing complete inhibition. Similarly, preincubation of neutrophils with 0-100 μmol/L genistein (tyrosine kinase inhibitor) also resulted in inhibition of OPZ- and Agg-IgG-induced MMP-9 secretion in a concentration-dependent manner, with 100 μmol/L genistein causing complete inhibition. Significant (p < 0.05) positive correlations were found between MMP-9 and luminal-dependent chemiluminescent response (LDCL) in the case of stimulation with OPZ (r = 0.754) and in the case of stimulation with Agg-IgG (r = 0.728). Our findings suggested that CR3 and FcR play a critical role in production of MMP-9 and may be regulated by intracellular signal transduction, including that by phosphoinositide 3-kinase (PI3K) and tyrosine kinase (TK). Springer Science + Business Media, Inc., 2007 neutrophil nationallicence matrix metalloproteinase-9 (MMP-9) nationallicence phosphoinositide 3-kinase (PI3K) nationallicence tyrosine kinase (TK) nationallicence complement receptor type 3 (CR3) nationallicence Fc receptor (FcR) nationallicence Agg-IgG : heat-aggregated IgG nationallicence CR3 : complement receptor type 3 nationallicence FcR : Fc receptor nationallicence LDCL : luminal-dependent chemiluminescent response nationallicence MMP-9 : matrix metalloproteinase-9 nationallicence OPZ : opsonized zymosan nationallicence PI3K : phosphoinositide 3-kinase nationallicence PKC : protein kinase C nationallicence TK : tyrosine kinase nationallicence Higuchi H. Animal Health Laboratory, Faculty of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan aut Ishizaka M. Animal Health Laboratory, Faculty of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan aut Nagahata H. Animal Health Laboratory, Faculty of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan aut Veterinary Research Communications Springer Netherlands 31/8(2007-11-01), 985-991 0165-7380 31:8<985 2007 31 11259 https://doi.org/10.1007/s11259-006-0201-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-0201-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Higuchi H. Animal Health Laboratory, Faculty of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan aut NATIONALLICENCE 700 1- Ishizaka M. Animal Health Laboratory, Faculty of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan aut NATIONALLICENCE 700 1- Nagahata H. Animal Health Laboratory, Faculty of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan aut NATIONALLICENCE 773 0- Veterinary Research Communications Springer Netherlands 31/8(2007-11-01), 985-991 0165-7380 31:8<985 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer