caa a22 4500 463238516 CHVBK 20180405153305.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s11259-007-0028-1 doi (NATIONALLICENCE)springer-10.1007/s11259-007-0028-1 The Mycobiota and Toxicity of Equine Feeds [Elektronische Daten] [K. Keller, B. Queiroz, L. Keller, J. Ribeiro, L. Cavaglieri, M. Pereyra, A. Dalcero, C. Rosa] Feed contamination can lead to nutrient losses and detrimental effects on animal health and production. The purposes of this study were to investigate the mycobiota in equine mixed feeds and to determine natural contamination with aflatoxin B1 (AFB1) and fumonisin B1 (FB1). Fungal enumeration of equine feed samples was done. A commercially available enzyme-linked immunosorbent assay kit was applied to quantify AFB1 and FB1. A comparison between ELISA and HPLC was carried out. Feed mould counts ranged from <1× 102 to 1× 105 cfu/g. The most frequent genus isolated was Aspergillus (40.54%), followed by Penicillium (18.38%) and Fusarium (16.22%). The most prevalent Aspergillus sp. was A. flavus (36%). AFB1 values ranged between 0.01 and 99.4 μg/kg. FB1 levels ranged between 0.01 and 7.49 μg/kg. HPLC and ELISA methods showed positive correlation for AFB1 and FB1 determinations (r = 0.9851 and r = 0.9791, respectively). The ELISA analytical method was efficient for AFB1 and FB1 detection. The scarcity of studies on natural fungal contamination and on the presence of AFB1 and FB1 in materials used as equine feed ingredients highlights the value and contribution of this study. Springer Science + Business Media, Inc., 2007 aflatoxin B1 nationallicence equine feed nationallicence fumonisin B1 nationallicence mycobiota nationallicence AFB1 : aflatoxin B1 nationallicence cfu : colony-forming units nationallicence DCPA : dichloran chloramphenicol peptone agar nationallicence DG18 : dichloran 18% glycerol agar nationallicence DRBC : dichloran rose bengal chloramphenicol agar nationallicence FB1 : fumonisin B1 nationallicence Keller K. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro, Brazil aut Queiroz B. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro, Brazil aut Keller L. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro, Brazil aut Ribeiro J. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro, Brazil aut Cavaglieri L. Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina aut Pereyra M. Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina aut Dalcero A. Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina aut Rosa C. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro, Brazil aut Veterinary Research Communications Springer Netherlands 31/8(2007-11-01), 1037-1045 0165-7380 31:8<1037 2007 31 11259 https://doi.org/10.1007/s11259-007-0028-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-0028-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Keller K. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Queiroz B. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Keller L. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Ribeiro J. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Cavaglieri L. Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina aut NATIONALLICENCE 700 1- Pereyra M. Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina aut NATIONALLICENCE 700 1- Dalcero A. Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina aut NATIONALLICENCE 700 1- Rosa C. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rio de Janeiro, Brazil aut NATIONALLICENCE 773 0- Veterinary Research Communications Springer Netherlands 31/8(2007-11-01), 1037-1045 0165-7380 31:8<1037 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer