caa a22 4500 463238540 CHVBK 20180405153305.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11259-007-3533-3 doi (NATIONALLICENCE)springer-10.1007/s11259-007-3533-3 Cloning and Sequence Analysis of the Gene Encoding LipL32 of Leptospira interrogans Serovar Sejroe [Elektronische Daten] [R. Amutha, P. Chaudhury, Amar Garg, P. Vasan, P. Cheema, S. Srivastava] Leptospira, a member of the order Spirochaetales, is the causative agent of leptospirosis, an important zoonosis encountered worldwide. The Leptospira interrogans serovar Sejroe was grown in EMJH medium and its DNA was isolated using standard techniques. The LipL32 gene was amplified using the reported primer of Kirschneri of LipL32. The amplified product was found to comprise 756 base pairs. This amplified gene fragment of LipL32 lipoprotein was cloned in E. coli (DH5α) cells using pDrive plasmid as a vector. The recombinant cells were selected on LB agar medium containing ampicillin, X-gal and isopropyl-β-d-thiogalactopyranoside. Plasmid was extracted from the recombinant white colonies, and restriction endonuclease (RE) analysis was carried out using PstI and SalI. On partial sequence analysis, the product exhibited 756 base pairs, corresponding to 251 amino acids. The cloned gene could be further used for expression of recombinant protein for serodiagnosis of leptospirosis. Springer Science + Business Media, Inc., 2007 leptospirosis nationallicence LipL32 cloning nationallicence EMJH : Ellinghausen-McCullough-Johnson-Harris nationallicence IPTG : Isopropyl-β-d-thiogalactopyranoside nationallicence LB : Luria-Bertani nationallicence OMP : outer membrane protein nationallicence PCR : polymerase chain reaction nationallicence RE : restriction enzyme nationallicence TBE : Tris borate EDTA nationallicence Amutha R. Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, India aut Chaudhury P. Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, India aut Garg Amar Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, India aut Vasan P. Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, India aut Cheema P. Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, India aut Srivastava S. Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, India aut Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 513-519 0165-7380 31:5<513 2007 31 11259 https://doi.org/10.1007/s11259-007-3533-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-3533-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Amutha R. Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, India aut NATIONALLICENCE 700 1- Chaudhury P. Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, India aut NATIONALLICENCE 700 1- Garg Amar Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, India aut NATIONALLICENCE 700 1- Vasan P. Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, India aut NATIONALLICENCE 700 1- Cheema P. Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, India aut NATIONALLICENCE 700 1- Srivastava S. Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, India aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 513-519 0165-7380 31:5<513 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer