caa a22 4500 463238559 CHVBK 20180405153305.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11259-007-3461-2 doi (NATIONALLICENCE)springer-10.1007/s11259-007-3461-2 The Effects of Salivary Gland Extracts from Boophilus microplus Ticks on Mitogen-stimulated Bovine Lymphocytes [Elektronische Daten] [C. Turni, R. Lee, L. Jackson] The effect of salivary gland extract (SGE) from the tick Boophilus microplus was examined in mitogen-stimulated lymphocytes in vitro. SGE was added to lymphocytes of seven cattle together with the mitogens concanavalin A (ConA), phytohaemagglutinin (PHA) and pokeweed mitogen (PWM). Semi-purified B cells from another seven cattle were stimulated with the mitogen lipopolysaccharide (LPS). PHA and ConA stimulated proliferation of lymphocytes to the same extent, but the inhibition due to SGE of Boophilus microplus on the proliferative response stimulated by PHA (39.0% ± 9.3%) was less than the inhibition of proliferative response stimulated by ConA (75.4% ± 6.9%). In contrast, SGE of B. microplus stimulated the proliferation of B cells in the presence of LPS in a dose-dependent manner. Enhanced stimulation of B cells by SGE at >4 μg in culture was greater than twice that observed when B cells were stimulated by LPS alone. SGE does not have a direct suppressive effect on bovine B cell proliferation; however, in vivo the effectiveness of B cell responses might be influenced by other immune factors, such as cytokine profiles. Springer Science + Business Media, Inc., 2007 Boophilus microplus nationallicence immunosuppression nationallicence lymphocytes nationallicence salivary gland nationallicence tick nationallicence BrdU : 5-bromo-2′-deoxyuridine nationallicence ConA : concanavalin A nationallicence LPS : lipopolysaccharide nationallicence PHA : phytohaemagglutinin nationallicence PWM : pokeweed mitogen nationallicence SGE : salivary gland extract nationallicence Turni C. Department of Microbiology and Parasitology, University of Queensland, St Lucia, Queensland aut Lee R. Centre of Infectious Diseases and Microbiology, Westmead Hospital, Westmead, New South Wales aut Jackson L. Department of Primary Industries and Fisheries, Biosecurity, Tick Fever Centre, Wacol, Queensland, Australia aut Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 545-552 0165-7380 31:5<545 2007 31 11259 https://doi.org/10.1007/s11259-007-3461-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-3461-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Turni C. Department of Microbiology and Parasitology, University of Queensland, St Lucia, Queensland aut NATIONALLICENCE 700 1- Lee R. Centre of Infectious Diseases and Microbiology, Westmead Hospital, Westmead, New South Wales aut NATIONALLICENCE 700 1- Jackson L. Department of Primary Industries and Fisheries, Biosecurity, Tick Fever Centre, Wacol, Queensland, Australia aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 545-552 0165-7380 31:5<545 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer