caa a22 4500 463238567 CHVBK 20180405153305.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11259-007-3551-1 doi (NATIONALLICENCE)springer-10.1007/s11259-007-3551-1 Elevated Plasma Levels of Interleukin 1β, Tumour Necrosis Factor α and Monocyte Chemotactic Protein 1 Are Associated with Pregnancy Toxaemia in Ewes [Elektronische Daten] [G. Yarım, S. Karahan, C. Nisbet] Pregnancy toxaemia is a metabolic disorder that results from an inadequate energy supply to the growing maternal-fetal unit. The mechanism underlying the pathogenesis of the syndrome has not been fully clarified; however, a key role for cytokines and chemokines including interleukin 1 β (IL-1β), tumour necrosis factor α (TNF-α) and monocyte chemotactic protein 1 (MCP-1) has been indicated in women and experimental animals. However, information on the maternal plasma levels of IL-1β, TNF-α and MCP-1 in ewes with pregnancy toxaemia is limited. Thus, the present study was designed to determine plasma IL-1β, TNF-α and MCP-1 concentrations in ewes with severe (n = 6) and mild (n = 4) naturally occurring pregnancy toxaemia and in uncomplicated pregnant ewes (n = 10) using enzyme-linked immunosorbent assay (ELISA). All ewes with pregnancy toxaemia had significantly lower body temperature and respiratory rate than uncomplicated pregnant ewes (p < 0.05). With the highest concentrations in severe cases, heart rate, proteinuria and serum uric acid levels as well as plasma IL-1β, TNF-α and MCP-1 were significantly different among all three groups (p < 0.05). The plasma concentrations of IL-1β in control ewes and ewes with mild and severe toxaemia were 15.81 ± 3.90 pg/ml, 23.83 ± 2.42 pg/ml and 34.55 ± 8.03 pg/ml, respectively. The plasma concentrations of TNF-α in control ewes and ewes with mild and severe toxaemia were 7.71 ± 1.61 pg/ml, 16.13 ± 3.63 pg/ml, and 22.85 ± 3.64 pg/ml, respectively. The plasma concentrations of MCP-1 in control ewes and ewes with mild and severe toxaemia were 101.70 ± 9.86 pg/ml, 134.75 ± 6.24 pg/ml, and 157.67 ± 9.69 pg/ml, respectively. Moreover, plasma IL-1β, TNF-α and MCP-1 levels were positively correlated with clinical and well-establish biochemical parameters of pregnancy toxaemia, serum uric acid and proteinuria (p < 0.01). Concomitant increase of plasma IL-1β, TNF-α and MCP-1 concentrations along with serum uric acid, proteinuria, and worsening of the clinical signs indicates that such cytokines are involved in the aetiopathogenesis and in perpetuation of the local and systemic inflammatory reactions in pregnancy toxaemia in ewes. Hence, plasma IL-1β, TNF-α and MCP-1 may potentially serve as markers to monitor prognosis of pregnancy toxaemia in ewes. Springer Science + Business Media, Inc., 2007 ewe nationallicence IL-1β nationallicence MCP-1 nationallicence plasma nationallicence pregnancy toxemia nationallicence TNF-α nationallicence EDTA : ethylenediaminetetraacetic acid nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence IL-1β : interleukin 1β nationallicence MCP-1 : monocyte chemotactic protein 1 nationallicence TNF-α : tumour necrosis factor α nationallicence Yarım G. Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey aut Karahan S. Department of Basic Sciences, Faculty of Veterinary Medicine, University of Kirikkale, Kirikkale, Turkey aut Nisbet C. Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey aut Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 565-573 0165-7380 31:5<565 2007 31 11259 https://doi.org/10.1007/s11259-007-3551-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-3551-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yarım G. Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey aut NATIONALLICENCE 700 1- Karahan S. Department of Basic Sciences, Faculty of Veterinary Medicine, University of Kirikkale, Kirikkale, Turkey aut NATIONALLICENCE 700 1- Nisbet C. Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 565-573 0165-7380 31:5<565 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer