caa a22 4500 463238575 CHVBK 20180405153305.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11259-007-3505-7 doi (NATIONALLICENCE)springer-10.1007/s11259-007-3505-7 Pharmacokinetic Profile of Erythromycin after Intramammary Administration in Lactating Dairy Cows with Specific Mastitis [Elektronische Daten] [N. Bajwa, B. Bansal, A. Srivastava, R. Ranjan] The pharmacokinetics of erythromycin was studied in five lactating dairy cows following single intramammary infusion of 300 mg erythromycin in each of two quarters per cow with specific mastitis. Levels of erythromycin in plasma and quarter milk samples were measured by agar plate diffusion assay using Micrococcus luteus (ATCC 9341) as the test organism. Erythromycin level in plasma reached a peak concentration value (C max) of 0.07 ± 0.01 μg/ml at 30 min; thereafter, levels declined gradually to reach 0.05 ± 0.00 μg/ml 12 h post drug administration. The pharmacokinetic profile of the drug revealed mean absorption half life (t 1/2ka) as 0.26 ± 0.05 h. The drug was eliminated slowly with elimination half-life (t 1/2β) of 13.75 ± 0.35 h and elimination rate constant (k el) of 0.04 ± 0.00 h−1. The volume of distribution based on the zero-time plasma concentration intercept of the least-squares regression line of the elimination phase (V d(B)) was 0.032 L/kg. The drug crossed to untreated quarters also; mean drug levels of 0.20 ± 0.07, 0.23 ± 0.07, 0.17 ± 0.04, and 0.17 ± 0.04 μg/ml were found at 3, 6, 8 and 12 h, respectively. The mean drug concentration for treated quarters was measured as 22.97 ± 2.31 μg/ml milk at first milking (12 h) following drug infusion. No apparent adverse reaction was seen in cows administered erythromycin. It is concluded that following intramammary infusion erythromycin diffuses readily and extensively in various body fluids and tissues and adequate concentration is maintained in udder tissues for at least 12 h post intramammary administration. Thus, erythromycin may be recommended for local therapy of acute mastitis caused by Gram-positive bacteria in lactating dairy cows. Springer Science + Business Media, Inc., 2007 cows nationallicence erythromycin nationallicence intramammary nationallicence pharmacokinetics nationallicence specific mastitis nationallicence ATCC : American Type Culture Collection nationallicence AUC0−∞ : area under the curve from zero to infinity by the trapezoidal integral nationallicence AUMC0−∞ : area under the moment curve nationallicence β : elimination rate constant nationallicence C 0 : zero-time plasma drug concentration intercept of the least-squares regression line of elimination phase nationallicence C max : peak plasma drug concentration value nationallicence IDF : International Dairy Federation nationallicence k a : absorption rate constant nationallicence k el : overall elimination rate constant nationallicence MIC : minimum inhibitory concentration nationallicence MRT : mean residual time nationallicence t 1/2ka : absorption half life nationallicence t 1/2β : elimination half-life nationallicence V d(B) : volume of distribution based on zero time plasma drug concentration intercept of the least-squares regression line of elimination phase nationallicence Bajwa N. Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, College of Veterinary Sciences, Punjab Agricultural University, Ludhiana, Punjab aut Bansal B. Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, College of Veterinary Sciences, Punjab Agricultural University, Ludhiana, Punjab aut Srivastava A. College of Veterinary Sciences, Jammu, India aut Ranjan R. Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, College of Veterinary Sciences, Punjab Agricultural University, Ludhiana, Punjab aut Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 603-610 0165-7380 31:5<603 2007 31 11259 https://doi.org/10.1007/s11259-007-3505-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-3505-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bajwa N. Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, College of Veterinary Sciences, Punjab Agricultural University, Ludhiana, Punjab aut NATIONALLICENCE 700 1- Bansal B. Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, College of Veterinary Sciences, Punjab Agricultural University, Ludhiana, Punjab aut NATIONALLICENCE 700 1- Srivastava A. College of Veterinary Sciences, Jammu, India aut NATIONALLICENCE 700 1- Ranjan R. Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, College of Veterinary Sciences, Punjab Agricultural University, Ludhiana, Punjab aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 603-610 0165-7380 31:5<603 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer