caa a22 4500 463238613 CHVBK 20180405153305.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11259-007-3501-y doi (NATIONALLICENCE)springer-10.1007/s11259-007-3501-y Reactivity of Equine Airways—A Study on Precision-cut Lung Slices [Elektronische Daten] [J. Vietmeier, F. Niedorf, W. Bäumer, C. Martin, E. Deegen, B. Ohnesorge, M. Kietzmann] A study was performed to evaluate the use of precision-cut lung slices (PCLS) for studies on the contraction of equine airways. Lungs of 10 horses were taken to prepare PCLS of ˜250 μm from equine lung tissue using a special microtome. The lung slices were cultured and the enclosed small airways were monitored using a microscope with coupled digital camera, which was used to determine the airway luminal area and diameter from digital images. As indicated by the beating of the ciliated epithelium and reactivity of airways on methacholine challenge, the tissue slices were found to be viable for at least 24 h. The airways were not precontracted, as indicated by a missing dilatory effect of 1 mmol/L clenbuterol. Bronchoconstriction induced by both methacholine and histamine was found to be dose dependent. EC50 values based on luminal area were 1.12 μmol/L · /÷ 3.82 for methacholine and 0.68 μmol/L · /÷ 6.99 for histamine. In conclusion, the PCLS technique is promising for studies on small airways in the equine lung. In the present study the basic principles of in vitro (ex vivo) examinations with equine PCLS on airway reactivity were developed. Springer Science + Business Media, Inc., 2007 histamine nationallicence horse nationallicence lung nationallicence methacholine nationallicence PCLS nationallicence COPD : chronic obstructive pulmonary disease nationallicence EC : effective concentration nationallicence MTT : dimethylthiazolyldiphenyltetrazolium bromide nationallicence PCLS : precision-cut lung slices nationallicence Vietmeier J. Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Hannover aut Niedorf F. Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Hannover aut Bäumer W. Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Hannover aut Martin C. Research Center Borstel, Germany aut Deegen E. Clinic for Horses, University of Veterinary Medicine Hannover, Hannover aut Ohnesorge B. Clinic for Horses, University of Veterinary Medicine Hannover, Hannover aut Kietzmann M. Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Hannover aut Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 611-619 0165-7380 31:5<611 2007 31 11259 https://doi.org/10.1007/s11259-007-3501-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-3501-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vietmeier J. Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Hannover aut NATIONALLICENCE 700 1- Niedorf F. Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Hannover aut NATIONALLICENCE 700 1- Bäumer W. Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Hannover aut NATIONALLICENCE 700 1- Martin C. Research Center Borstel, Germany aut NATIONALLICENCE 700 1- Deegen E. Clinic for Horses, University of Veterinary Medicine Hannover, Hannover aut NATIONALLICENCE 700 1- Ohnesorge B. Clinic for Horses, University of Veterinary Medicine Hannover, Hannover aut NATIONALLICENCE 700 1- Kietzmann M. Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Hannover aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 611-619 0165-7380 31:5<611 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer